Sarsuag Enterprises
Obeetee Textiles Pvt. Ltd. Interview Questions and Answers
Q1. Name the E. coli strain used for protein expression
BL21(DE3) is a commonly used E. coli strain for protein expression.
BL21(DE3) is a popular E. coli strain for protein expression due to its ability to efficiently produce recombinant proteins.
It is a derivative of E. coli B strain and contains the T7 RNA polymerase gene under the control of the lacUV5 promoter.
BL21(DE3) is often used in conjunction with pET plasmids for protein expression in research laboratories.
Q2. Differencr between complete and incomplete adjuvant
Complete adjuvant contains killed or inactivated pathogens along with an immunostimulant, while incomplete adjuvant lacks the pathogen component.
Complete adjuvant includes killed or inactivated pathogens and an immunostimulant
Incomplete adjuvant lacks the pathogen component
Complete adjuvant is more potent in inducing immune response compared to incomplete adjuvant
Q3. What are biosminilars. Give an example
Biosimilars are biological products that are highly similar to a reference product, with no clinically meaningful differences in terms of safety and efficacy.
Biosimilars are not exact copies of the original biologic drug, but are highly similar.
They are approved based on demonstrating similarity to the reference product in terms of quality, safety, and efficacy.
Examples of biosimilars include filgrastim-sndz (Zarxio) which is a biosimilar to filgrastim (Neupogen).
Q4. Define pharmacodynamics and pharmacokinetics
Pharmacodynamics is the study of the effects of drugs on the body, while pharmacokinetics is the study of how the body affects the drug.
Pharmacodynamics focuses on the mechanism of action of drugs and their effects on the body.
Pharmacokinetics deals with the absorption, distribution, metabolism, and excretion of drugs in the body.
An example of pharmacodynamics is studying how a drug binds to a receptor to produce a therapeutic effect.
An example of pharmacokinetics is analyzin...read more
Q5. Are u interested in phD
Yes, I am interested in pursuing a PhD in the future.
I have a strong passion for research and a desire to further my knowledge in a specific field.
I believe a PhD will provide me with the necessary skills and expertise to make significant contributions to the field.
I am excited about the opportunity to conduct in-depth research and contribute to the academic community.
Q6. Example of gram negative bacteria
Gram negative bacteria have a thin peptidoglycan layer and an outer membrane containing lipopolysaccharides.
Escherichia coli
Pseudomonas aeruginosa
Salmonella typhi
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