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Obeetee Textiles Pvt. Ltd. Interview Questions and Answers

Updated 1 Sep 2024

Q1. Name the E. coli strain used for protein expression

Ans.

BL21(DE3) is a commonly used E. coli strain for protein expression.

  • BL21(DE3) is a popular E. coli strain for protein expression due to its ability to efficiently produce recombinant proteins.

  • It is a derivative of E. coli B strain and contains the T7 RNA polymerase gene under the control of the lacUV5 promoter.

  • BL21(DE3) is often used in conjunction with pET plasmids for protein expression in research laboratories.

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Q2. Differencr between complete and incomplete adjuvant

Ans.

Complete adjuvant contains killed or inactivated pathogens along with an immunostimulant, while incomplete adjuvant lacks the pathogen component.

  • Complete adjuvant includes killed or inactivated pathogens and an immunostimulant

  • Incomplete adjuvant lacks the pathogen component

  • Complete adjuvant is more potent in inducing immune response compared to incomplete adjuvant

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Q3. What are biosminilars. Give an example

Ans.

Biosimilars are biological products that are highly similar to a reference product, with no clinically meaningful differences in terms of safety and efficacy.

  • Biosimilars are not exact copies of the original biologic drug, but are highly similar.

  • They are approved based on demonstrating similarity to the reference product in terms of quality, safety, and efficacy.

  • Examples of biosimilars include filgrastim-sndz (Zarxio) which is a biosimilar to filgrastim (Neupogen).

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Q4. Define pharmacodynamics and pharmacokinetics

Ans.

Pharmacodynamics is the study of the effects of drugs on the body, while pharmacokinetics is the study of how the body affects the drug.

  • Pharmacodynamics focuses on the mechanism of action of drugs and their effects on the body.

  • Pharmacokinetics deals with the absorption, distribution, metabolism, and excretion of drugs in the body.

  • An example of pharmacodynamics is studying how a drug binds to a receptor to produce a therapeutic effect.

  • An example of pharmacokinetics is analyzin...read more

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Q5. Are u interested in phD

Ans.

Yes, I am interested in pursuing a PhD in the future.

  • I have a strong passion for research and a desire to further my knowledge in a specific field.

  • I believe a PhD will provide me with the necessary skills and expertise to make significant contributions to the field.

  • I am excited about the opportunity to conduct in-depth research and contribute to the academic community.

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Q6. Example of gram negative bacteria

Ans.

Gram negative bacteria have a thin peptidoglycan layer and an outer membrane containing lipopolysaccharides.

  • Escherichia coli

  • Pseudomonas aeruginosa

  • Salmonella typhi

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