Palamur Biosciences Research Assistant Interview Questions and Answers

The primary structure of a protein is the linear sequence of amino acids in a polypeptide chain.

• The primary structure determines the protein's shape and function.

• It is determined by the DNA sequence of the gene that codes for the protein.

• Protein molecular weight and size can be determined by techniques such as gel electrophoresis, mass spectrometry, and size exclusion chromatography.

• For example, gel electrophoresis sep...read more

SDS PAGE stands for Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis.

• It is a technique used to separate proteins based on their size and charge.

• SDS is a detergent that denatures the proteins and gives them a negative charge.

• The proteins are then separated based on their size as they move through the gel matrix.

• It is commonly used in biochemistry and molecular biology research.

• Example: SDS PAGE can be used to a...read more