Lupin Quality Analyst Interview Questions and Answers

HPLC principle is based on the separation of components in a mixture using a stationary phase and a mobile phase.

• HPLC stands for High Performance Liquid Chromatography

• It is a technique used to separate, identify, and quantify components in a mixture

• The principle involves passing a mixture through a column containing a stationary phase, which separates the components based on their interactions with the stationary phase

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UV wavelength ranges from 10 nm to 400 nm.

• UV-A: 315-400 nm

• UV-B: 280-315 nm

• UV-C: 100-280 nm

• UV-Vacuum: 10-200 nm

UV wavelength range is between 10 nm to 400 nm.

• UV range is divided into three categories: UV-A (315-400 nm), UV-B (280-315 nm), and UV-C (100-280 nm).

• UV-C is the most harmful to living organisms and is mostly absorbed by the Earth's atmosphere.

• UV radiation is used in various fields such as sterilization, curing, and forensic analysis.

The standard operating procedure of FT-IR analysis involves sample preparation, instrument calibration, data acquisition, and interpretation.

• Sample preparation involves obtaining a representative sample and preparing it for analysis.

• Instrument calibration ensures accurate measurements by calibrating the instrument using known reference materials.

• Data acquisition involves placing the sample in the FT-IR instrument and c...read more