International Center for Agricultural Research in the Dry Areas Research Associate Interview Questions and Answers for Experienced

I would investigate the cause and try alternative sterilization methods.

• Check if there is an issue with the equipment or the sterilization process

• Try alternative sterilization methods such as chemical sterilization or dry heat sterilization

• Consult with colleagues or experts in the field for advice

• Document the issue and any actions taken for future reference

Shake flask study is a method used to evaluate the growth and productivity of microorganisms in a small-scale culture.

• It involves growing microorganisms in a small flask with shaking to provide oxygen and nutrients.

• The flask is incubated at a specific temperature and pH to mimic the conditions of the larger culture.

• Samples are taken at regular intervals to measure cell growth and product formation.

• Shake flask studies a...read more

Glycerol is used for long term preservation of bacterial cultures due to its ability to prevent ice crystal formation and cell damage.

• Glycerol acts as a cryoprotectant, preventing ice crystal formation and cell damage during freezing.

• It allows for long term storage of bacterial cultures at -80°C.

• Glycerol is added to the bacterial culture before freezing to a final concentration of 15-20%.

• It is a common practice in micr...read more

I would choose to follow safety parameters over GLP.

• Safety of personnel and environment is of utmost importance

• GLP can be compromised temporarily but safety cannot

• Non-compliance with safety regulations can lead to serious consequences

The two most important reactions for humans, animals and plants are photosynthesis and respiration.

• Photosynthesis is the process by which plants convert sunlight, carbon dioxide and water into glucose and oxygen.

• Respiration is the process by which living organisms convert glucose and oxygen into energy, carbon dioxide and water.

• Photosynthesis and respiration are complementary processes that sustain life on Earth.

• Photos...read more

My 12th marks were affected due to personal reasons, but I have consistently performed well in other academic pursuits.

• I faced some personal challenges during my 12th grade which affected my performance.

• However, I have consistently performed well in other academic pursuits such as my graduation.

• My 10th grade marks are a reflection of my academic potential and I have worked hard to maintain that level of performance.

• I h...read more

Answering questions related to upstream processing, including process flow, material and system use, FIT, CIP, SIP, bioreactor system, and role of raw material in media.

• Previous job responsibilities in upstream processing

• Process flow of upstream processing

• Make and MOC of the material and system use during operation

• FIT, CIP, and SIP

• Types of and part of bioreactor system

• Role of raw material in media

A 50 ppm solution contains 50 parts of solute per million parts of solution.

• ppm stands for parts per million.

• It is a measure of concentration.

• In a 50 ppm solution, there are 50 parts of solute per million parts of solution.

• For example, if we have a 1 million mL solution, there would be 50 mL of solute in it.