Genesys Biologics Interview Questions and Answers for Experienced

Column efficiency can be evaluated by measuring the resolution, peak asymmetry, and plate count.

• Resolution can be calculated by dividing the distance between two peaks by the sum of their peak widths.

• Peak asymmetry can be determined by measuring the distance between the peak maximum and the point where the slope of the peak is half of the maximum slope.

• Plate count can be calculated by dividing the column length by the

Ion exchange chromatography separates molecules based on their charge.

• Ion exchange chromatography uses a resin with charged functional groups to attract and bind molecules with opposite charges.

• The bound molecules can then be eluted by changing the pH or salt concentration of the solution.

• This technique is commonly used in downstream processing to purify proteins and other biomolecules.

• Examples of ion exchange resins i...read more

Packing and unpacking a column involves filling and emptying the column with the desired material.

• Packing the column involves adding the desired material to the column and ensuring it is evenly distributed.

• Unpacking the column involves removing the material from the column, usually by flushing it with a suitable solvent.

• The packing material used depends on the type of column and the desired separation.

• Common packing ma...read more

The chromatography software and version used is dependent on the specific process and equipment being utilized.

• The software and version may vary depending on the manufacturer of the chromatography equipment.

• Common chromatography software includes Unicorn, Chromeleon, and Empower.

• The version used may also depend on the age of the equipment and compatibility with newer software.

• The software is used to control the chromat

Flux in TFF refers to the rate of flow of the liquid through the membrane during filtration.

• Flux is measured in volume per unit area per unit time.

• It is influenced by factors such as pressure, temperature, and membrane pore size.

• High flux rates can lead to membrane fouling and reduced filtration efficiency.

NWP stands for nominal molecular weight cut-off in TFF.

• NWP is a parameter used in tangential flow filtration (TFF) to determine the size of molecules that can pass through the membrane.

• It is the molecular weight at which 90% of the molecules are retained by the membrane.

• NWP is important in downstream processing to ensure the desired molecules are separated from impurities.

• For example, if the target molecule has a molec...read more

Column volume can be calculated by dividing the total bed volume by the packing factor.

• Determine the total bed volume of the column

• Calculate the packing factor of the column

• Divide the total bed volume by the packing factor to get the column volume

Volumetric flow rate is the volume of fluid passing through a given point per unit time, while linear flow rate is the distance traveled by the fluid per unit time.

• Volumetric flow rate is measured in units of volume per unit time, such as liters per minute or gallons per hour.

• Linear flow rate is measured in units of distance per unit time, such as meters per second or feet per minute.

• Volumetric flow rate is used to des...read more