CSIR-CCMB Interview Questions and Answers

Positive dominant mutation in Lac Operon enhances the expression of genes involved in lactose metabolism.

• Positive dominant mutation increases the activity of the Lac Operon

• This mutation leads to increased expression of genes involved in lactose metabolism

• Examples of positive dominant mutations include LacI+ and CRP+

PCR is a technique to amplify DNA. Hot start method is a modification to prevent non-specific amplification.

• PCR involves denaturation, annealing, and extension steps

• Hot start method involves adding Taq polymerase after initial denaturation to prevent non-specific amplification

• Hot start can be achieved by using modified Taq polymerase or by adding inhibitors like antibodies or wax beads

• Hot start PCR is useful for amplif...read more