Glean Interview Questions and Answers

Different types of detectors for HPLC include UV-Vis, fluorescence, refractive index, and mass spectrometry.

• UV-Vis detector measures the absorbance of UV or visible light by the analyte.

• Fluorescence detector measures the fluorescence emitted by the analyte when excited by light.

• Refractive index detector measures the change in refractive index of the mobile phase caused by the analyte.

• Mass spectrometry detector measures the mass-to-charge ratio of ions produced by the analyte.

The compounds that were ELSD inactive are those that did not produce a response in the ELSD detector.

• ELSD inactive compounds do not show any signal or peak in the ELSD detector.

• These compounds may have low or no response to the ELSD detection method.

• ELSD inactive compounds can be identified by comparing their chromatographic profiles to those of active compounds.

• Examples of ELSD inactive compounds include certain sugars, salts, and some non-volatile compounds.

Suzuki coupling byproduct is typically a biphenyl compound. LCMS shows peaks corresponding to reactants, products, and intermediates.

• Suzuki coupling byproduct is often a biphenyl compound formed due to side reactions.

• LCMS analysis of Suzuki reaction typically shows peaks corresponding to reactants, products, and intermediates.

• Monitoring the LCMS peaks can provide insights into the progress and efficiency of the Suzuki coupling reaction.

Nitrile reacts with lithium aluminum hydride to form primary amines, and with DiBaL-H to form aldehydes.

• Nitrile + lithium aluminum hydride -> primary amine

• Nitrile + DiBaL-H -> aldehyde

Conforming cis and trans isomers by NMR involves analyzing the chemical shifts and coupling constants of the molecules.

• Analyze the chemical shifts of the protons in the molecule to determine the stereochemistry (cis or trans).

• Look for characteristic coupling constants that indicate the relative positions of the protons in the molecule.

• Compare the NMR spectra of the cis and trans isomers to identify any differences in chemical shifts or coupling constants.

• Use 2D NMR techniques...read more

I expect a competitive annual CTC based on my experience, skills, and market standards.

• Expectation of a salary package that reflects my qualifications and experience

• Consideration of market standards and industry norms

• Opportunity for performance-based bonuses or incentives

• Room for negotiation based on additional benefits like healthcare, retirement plans, etc.