Aragen Life Sciences Research Analyst Interview Questions and Answers

N-alkylation is a process of adding an alkyl group to a primary or secondary amine.

• N-alkylation can be achieved through various methods such as reductive amination, Gabriel synthesis, and Hoffmann degradation.

• Primary amines can be selectively N-alkylated using alkyl halides and a base, while secondary amines require a more reactive alkylating agent such as an epoxide or an alkyl halide.

• N-alkylation is an important reac...read more

Understanding reaction mechanisms, principles of HPLC NMR, and writing synthetic schemes for target molecules.

• Reaction mechanisms involve understanding the step-by-step process by which reactants are transformed into products.

• HPLC (High Performance Liquid Chromatography) is a technique used for separating compounds in a mixture based on their interactions with a stationary phase and a mobile phase.

• NMR (Nuclear Magnetic...read more

The reagent used for acid-amine coupling is typically carbodiimides.

• Carbodiimides are commonly used reagents for acid-amine coupling reactions.

• Examples of carbodiimides include EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) and DIC (diisopropylcarbodiimide).

Pyrophoric reagents can be quenched by using appropriate quenching agents.

• Use a non-reactive quenching agent such as water, alcohol, or mineral oil.

• Add the quenching agent slowly to the pyrophoric reagent while stirring gently.

• Dispose of the quenched mixture according to safety guidelines.

• Always wear appropriate personal protective equipment when handling pyrophoric reagents.