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Validation of GC and HPLC involves testing and verifying the accuracy and reliability of the instruments and methods used for analysis.
Validation ensures that the instruments and methods used for analysis are accurate and reliable.
It involves testing the instruments and methods using known standards and samples.
Validation parameters include accuracy, precision, linearity, specificity, and robustness.
Validation is neces...
Calibration GC & HPLC is the process of adjusting and verifying the accuracy of the instruments to ensure reliable and precise results.
Calibration is necessary to ensure accurate and precise measurements
GC and HPLC are analytical instruments used in chemical analysis
Calibration involves adjusting the instrument to match known standards
Verification of accuracy is done by comparing the instrument's readings to known stan...
Troubleshooting GC & HPLC involves identifying and resolving issues with the instruments and analysis process.
Trubal shooting GC & HPLC is the process of identifying and resolving issues with gas chromatography and high-performance liquid chromatography instruments and analysis.
Common issues include poor peak shape, baseline noise, and retention time shifts.
Troubleshooting may involve adjusting instrument parameters, c...
Adit try is not a known term in chemistry or any other field.
Adit try is not a recognized term in any field.
It is possible that the interviewer misspoke or meant to ask a different question.
Without further context, it is impossible to provide a more specific answer.
RRT stands for Relative Retention Time, a measure of the separation between two compounds in a chromatography column.
RRT is calculated by dividing the retention time of a compound by the retention time of a reference compound.
It is used to identify and quantify compounds in a mixture.
RRT values are specific to the chromatography system and conditions used.
For example, RRT can be used to distinguish between isomers with
GC and HPLC are analytical techniques used in chemistry to separate, identify, and quantify components in a mixture.
GC stands for Gas Chromatography and HPLC stands for High Performance Liquid Chromatography.
Both techniques involve passing a mixture through a stationary phase and a mobile phase to separate its components based on their physical and chemical properties.
GC is used for volatile and semi-volatile compounds...
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posted on 2 Jan 2025
I was interviewed before Jan 2024.
Calibration process for UV and HPLC instruments involves setting up reference standards, adjusting parameters, and verifying accuracy.
Prepare reference standards for UV and HPLC analysis
Adjust wavelength, detector sensitivity, and flow rate for UV instrument
Run standard solutions to verify accuracy and make necessary adjustments
Perform system suitability tests to ensure instrument performance meets requirements
Reverse phase chromatography uses a non-polar stationary phase and a polar mobile phase, while normal phase chromatography uses a polar stationary phase and a non-polar mobile phase.
Reverse phase chromatography is more commonly used in analytical chemistry for separating non-polar compounds, while normal phase chromatography is used for polar compounds.
In reverse phase chromatography, the more non-polar compounds elute...
I applied via Naukri.com and was interviewed in Oct 2023. There were 2 interview rounds.
CAPA flow involves identifying deviations, investigating root causes, implementing corrective actions, and verifying effectiveness.
Identify the deviation or nonconformance
Investigate the root cause using techniques like 5 Whys, Fishbone diagram
Implement corrective actions to prevent recurrence
Verify the effectiveness of the actions taken
Document the entire CAPA process for future reference
I applied via Naukri.com and was interviewed before Nov 2023. There were 2 interview rounds.
Reagents should be handled with care and proper safety measures to avoid accidents and contamination.
Always wear appropriate personal protective equipment (PPE) such as gloves, goggles, and lab coat.
Read and follow the instructions on the label or Material Safety Data Sheet (MSDS) before using any reagent.
Store reagents properly in designated areas and keep them away from incompatible substances.
Dispose of reagents pro...
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