10+ Research Scientist 1 Interview Questions and Answers

Solubility for dissolution and sink condition can be determined by conducting solubility studies and using appropriate sink conditions.

• Conduct solubility studies using various solvents and methods such as shake-flask method or UV spectroscopy.

• Ensure sink conditions are maintained by using a large volume of dissolution medium compared to the amount of drug being dissolved.

• Monitor the concentration of the drug over time to determine the solubility profile.

• Calculate the solubili...read more

The procedure for developing assays, dissolution, and related substances involves method validation and optimization for accurate results.

• Define the purpose of the assay, such as quantifying active ingredients.

• Select appropriate analytical techniques (e.g., HPLC, UV-Vis spectroscopy).

• Develop a dissolution method that mimics physiological conditions.

• Establish criteria for related substances, including limits and detection methods.

• Validate the method for specificity, accuracy, ...read more

Plant cell culture involves growing plant cells in a controlled environment, while animal cell culture involves growing animal cells.

• Plant cell culture is typically done in a solid or liquid medium containing nutrients and growth hormones.

• Animal cell culture is often done in a similar medium, but may require additional factors such as specific growth factors or hormones.

• Plant cells have a rigid cell wall, while animal cells do not.

• Plant cell culture is commonly used for study...read more

Discussing the reporting of molecular and serological testing of viral samples in research

• Molecular testing involves detecting the genetic material of the virus, such as PCR tests

• Serological testing looks for antibodies produced by the body in response to the virus

• Reporting should include details on the methods used, results obtained, and interpretation of findings

• Examples of molecular tests include RT-PCR and sequencing

• Examples of serological tests include ELISA and Western ...read more

Different types of APIs can be synthesized through various chemical reactions and processes.

• Identify the desired API structure and functional groups

• Select appropriate starting materials and reagents

• Plan and execute synthesis route using suitable reactions such as condensation, reduction, oxidation, etc.

• Purify and characterize the synthesized API through techniques like chromatography, spectroscopy, and crystallography

Hematoxylin and eosin (H&E) dye is commonly used in staining for histology and pathology purposes.

• Hematoxylin is a basic dye that stains nuclei blue-purple.

• Eosin is an acidic dye that stains cytoplasm and extracellular matrix pink or red.

• H&E staining is widely used in histology to visualize tissue structures and abnormalities.

Serological testing of viral samples involves detecting antibodies produced by the immune system in response to a viral infection.

• Serological testing is used to determine if a person has been exposed to a specific virus.

• It can also help in identifying past infections and immunity levels.

• Examples of serological tests include ELISA, Western blot, and rapid antibody tests.

Cell culture is the process of growing cells outside of their natural environment in a controlled setting.

• Cells are typically grown in a sterile environment with specific nutrients and conditions to support their growth

• Cell culture is commonly used in research to study cell behavior, drug testing, and disease modeling

• Examples of cell culture techniques include adherent cell culture, suspension cell culture, and primary cell culture

RT-PCR is a molecular technique used to detect the presence of SARS-CoV-2 virus in a sample.

• RT-PCR stands for reverse transcription polymerase chain reaction

• It is used to amplify and detect specific RNA sequences, such as those from SARS-CoV-2

• RT-PCR is a sensitive and specific method for diagnosing viral infections

• Results are typically reported as positive or negative for the virus

• Example: RT-PCR testing is commonly used for diagnosing COVID-19