Quality Control Microbiologist Interview Questions and Answers for Freshers

Autoclave validation is done by physical and biological methods to ensure proper sterilization.

• Physical methods include temperature and pressure monitoring.

• Biological methods include spore testing using Bacillus stearothermophilus or Geobacillus stearothermophilus.

• Validation should be done initially, after any repairs or maintenance, and periodically thereafter.

• Validation records should be maintained for regulatory compliance.

Autoclaves are used for sterilization. There are different types of autoclaves based on their usage and design.

• Gravity displacement autoclave

• Pre-vacuum autoclave

• Steam-flush pressure-pulse autoclave

• High-speed pre-vacuum sterilizer

• Tabletop autoclave

• Vertical autoclave