Cell Culture Analyst Interview Questions and Answers

Challenges of 3D cell culture include maintaining cell viability, reproducibility, and scalability.

• Diffusion limitations can lead to necrosis in the center of the culture

• Controlling the microenvironment is crucial for cell differentiation and function

• Developing appropriate scaffolds and matrices for cell growth

• Standardization of protocols for reproducibility

• Difficulty in scaling up for commercial production

• Increased complexity and cost compared to traditional 2D culture

• Limite...read more

Maintain sterile environment, handle cells carefully, use proper equipment and media, monitor regularly.

• Wear appropriate protective gear

• Disinfect surfaces and equipment

• Avoid opening culture vessels unnecessarily

• Use sterile techniques when handling cells

• Monitor cultures regularly for signs of contamination