Bioinformatics Analyst Interview Questions and Answers

Various databases are used for population genetics research, including dbSNP, 1000 Genomes Project, and HapMap.

• dbSNP: a database of single nucleotide polymorphisms (SNPs) maintained by the National Center for Biotechnology Information (NCBI)

• 1000 Genomes Project: a public database of genetic variation in over 2,500 individuals from 26 populations worldwide

• HapMap: a database of common genetic variations in human populations, used to identify genetic factors associated with dise...read more

I am familiar with various file formats used in bioinformatics.

• FASTA

• FASTQ

• SAM/BAM

• VCF

• BED

• GTF/GFF

• WIG/BigWig

• BAM/SAM

• CRAM

• BLAST

I am familiar with various databases including GenBank, UniProt, NCBI, and PDB.

• GenBank - a genetic sequence database maintained by NCBI

• UniProt - a protein sequence and function database

• NCBI - a collection of biomedical databases including GenBank and PubMed

• PDB - a database of 3D structures of proteins and nucleic acids

NGS pipeline involves steps like quality control, alignment, variant calling, annotation, and interpretation.

• Quality control of raw data to remove low-quality reads and adapters

• Alignment of reads to a reference genome or transcriptome

• Variant calling to identify genetic variations

• Annotation of variants to determine their functional impact

• Interpretation of results to draw conclusions about the biological significance of the variants

• Examples of tools used in NGS pipeline are Fas...read more

NGS data analysis involves processing and interpreting large amounts of sequencing data to extract meaningful biological insights.

• NGS data analysis involves quality control, alignment, variant calling, and downstream analysis.

• Tools like BWA, GATK, and SAMtools are commonly used for NGS data analysis.

• Common analyses include identifying genetic variants, gene expression levels, and pathway analysis.

• NGS data analysis can be used in genomics, transcriptomics, epigenomics, and met...read more