Walk-In Interview @ Pune - Cell and Gene Therapy - R&D & Manufacturing

Lentiviral vector mfg process development

• Conducting upstream and downstream processes for lentivirus production.
• Preparing media and reagents.
• Generation of cell banks, reviving and maintenance of various mammalian cell lines.
• Performing transient and stable transfections for lentivirus generation.
• Optimizing processes to achieve high titers.
• Scaling up production in cell factories or bioreactors.
• Purifying and concentrating lentivirus using tangential flow/diafiltration and chromatography methods.
• Recording data and preparing regulatory documents.

CAR-T (Chimeric Antigen Receptor T cell)  Manufacturing:

• Executing CAR-T cell production at lab scale and/or with automated/semi-automated systems.
• Preparing media and reagents.
• Handling human blood and cells.
• Isolating immune cells (PBMCs/T cells) from blood.
• Transducing cells with lentivirus.
• Expanding cells, formulating them, and cryopreserving.
• Reviving and maintaining cells.
• Recording data and preparing regulatory documents.

Quality Control:

• Performing in-process quality control (IPQC) and release tests for CAR-T and lentivirus
• Preparing media and reagents.
• Conducting FACS-based analyses for cell characterization.
• Performing molecular biology assays (RT-PCR/qPCR, agarose gel electrophoresis, DNA sequencing) for characterizing lentivirus and CAR-T cells.
• Conducting cell-based assays for potency testing.
• Performing immunoassays for cytokine profiling.
• Validation/qualification of methods
• Recording data and preparing regulatory documents.Role & responsibilities

Preferred candidate profile

Qualifications/Experience PhD, M.Tech, or M.Sc in Life Sciences, Biochemistry, Biotechnology, or Microbiology.

Freshers or candidates with 2-8 years of experience in Cell and Gene Therapy (CGT).

Perks and benefits