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Normal phase chromatography is a type of liquid chromatography where the stationary phase is polar and the mobile phase is nonpolar.
Stationary phase is polar
Mobile phase is nonpolar
Used to separate polar compounds
Commonly used in the separation of lipids and fatty acids
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posted on 12 Jul 2024
To integrate an HPLC graph, identify peaks, set baseline, calculate area under peaks, and sum all peak areas.
Identify peaks by setting appropriate detection parameters.
Set baseline by adjusting baseline correction.
Calculate area under peaks using integration software.
Sum all peak areas to get total area under the curve.
HPLC columns are used in chromatography to separate and analyze compounds based on their interactions with the stationary phase.
There are different types of HPLC columns such as reverse-phase, normal-phase, ion-exchange, size-exclusion, and affinity columns.
Reverse-phase columns are the most commonly used and separate compounds based on hydrophobic interactions.
Normal-phase columns separate compounds based on polarity.
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Chromatography is a technique used to separate and analyze mixtures of substances based on their different properties.
Chromatography involves a stationary phase and a mobile phase.
The stationary phase can be a solid or a liquid that is fixed in place.
The mobile phase is a liquid or gas that carries the mixture through the stationary phase.
Different components of the mixture interact differently with the stationary phas...
Normality refers to the concentration of a substance in a solution, expressed as the number of equivalents per liter.
Normality is a measure of the concentration of a substance in a solution.
It is expressed as the number of equivalents per liter (eq/L).
Normality is commonly used in acid-base reactions and redox reactions.
For example, a 1M solution of hydrochloric acid (HCl) is also 1N because HCl is monoprotic.
However, ...
Molality is a measure of the concentration of a solute in a solvent, expressed as the number of moles of solute per kilogram of solvent.
Molality is different from molarity, which is the number of moles of solute per liter of solution.
It is commonly used in chemistry and pharmacy to accurately measure the concentration of a solute in a solvent.
Molality is temperature-independent, unlike molarity.
For example, a solution ...
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