Cogent E Services Interview Questions and Answers

Common contaminations in cell culture include bacterial, fungal, viral, and mycoplasma contaminations.

• Bacterial contamination: Evidenced by turbidity, pH changes, and presence of biofilms.

• Fungal contamination: Visible as fuzzy growth or discoloration in the culture.

• Viral contamination: Detected through cytopathic effects or specific viral assays.

• Mycoplasma contamination: Difficult to detect visually, requires PCR or DNA staining methods.

Yes, I have experience in primary tumor cell culture.

• I have worked with primary tumor cells in a laboratory setting.

• I am familiar with the techniques and protocols involved in primary tumor cell culture.

• I have successfully cultured primary tumor cells for various experiments and assays.

• I have experience in maintaining the viability and growth of primary tumor cells in culture.

• I am knowledgeable about the challenges and considerations specific to primary tumor cell culture.

Yes, I know how to culture primary tumor cells.

• Primary tumor cells can be cultured by isolating them from the tumor tissue.

• The cells are then placed in a suitable culture medium that provides the necessary nutrients and growth factors.

• The culture conditions, such as temperature, humidity, and CO2 levels, are optimized to support cell growth.

• Regular monitoring and maintenance of the culture are required to ensure cell viability and prevent contamination.

• Primary tumor cells can...read more

Quality control criteria for lab work include accuracy, precision, reliability, and adherence to standard protocols.

• Accuracy: Ensuring that the lab results are close to the true value.

• Precision: Consistency and reproducibility of results when the same sample is tested multiple times.

• Reliability: The ability of the lab to consistently produce accurate and precise results.

• Adherence to standard protocols: Following established procedures and guidelines to maintain consistency an...read more

Yes, mycoplasma can be seen with a microscope.

• Mycoplasma is a type of bacteria that lacks a cell wall, making it difficult to stain and visualize under a microscope.

• However, it can be observed using specialized staining techniques such as fluorescent dyes or electron microscopy.

• Mycoplasma infections are commonly diagnosed by examining samples of body fluids or tissues under a microscope.

• For example, a sputum sample from a patient with respiratory symptoms can be stained and e...read more

I expect a competitive salary based on industry standards and my qualifications.

• I am looking for a salary that reflects my skills and experience in the field.

• I have researched the average salary range for Junior Scientists in this industry.

• I am open to negotiation based on the overall compensation package offered.

• I value opportunities for growth and development in addition to monetary compensation.

To complete the validation of an abnormal test result, follow these steps:

• Review the patient's medical history and any relevant clinical information

• Check for any potential errors or issues with the testing process

• Compare the abnormal result with previous test results for the same patient

• Consult with other healthcare professionals or specialists for their input

• Perform additional confirmatory tests if necessary

• Document the validation process and any actions taken

The methods used by this instrument include PCR, ELISA, and immunohistochemistry.

• Polymerase Chain Reaction (PCR) is used to amplify DNA sequences.

• Enzyme-Linked Immunosorbent Assay (ELISA) is used to detect and quantify proteins or antibodies.

• Immunohistochemistry is used to visualize specific antigens in tissue sections.