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10+ Crown Builders Interview Questions and Answers

Updated 13 May 2024

Q1. What is the pH of 10 to the power -8 Molar HCL?

Ans.

The pH of 10^-8 Molar HCL is 8.

  • pH is a measure of the acidity or alkalinity of a solution.

  • The pH scale ranges from 0 to 14, with 7 being neutral.

  • A pH below 7 indicates acidity, while a pH above 7 indicates alkalinity.

  • The pH of a solution can be calculated using the formula pH = -log[H+].

  • In this case, the concentration of H+ ions is 10^-8 Molar, so the pH is 8.

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Q2. Three most important reasons why DNA is the genetic material?

Ans.

DNA is the genetic material due to its ability to store, replicate, and transmit genetic information.

  • DNA can store vast amounts of genetic information in its sequence of nucleotides.

  • DNA can replicate itself accurately, ensuring the transmission of genetic information to offspring.

  • DNA can transmit genetic information from one generation to the next through the process of inheritance.

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Q3. how will you differentiate without wasting the sample much?

Ans.

By using efficient testing methods and techniques, we can differentiate without wasting the sample much.

  • Utilize non-destructive testing methods

  • Implement statistical analysis to minimize sample size

  • Utilize advanced technology for accurate and precise measurements

  • Optimize testing procedures to reduce sample consumption

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Q4. Which molecule does glycolysis start with?

Ans.

Glycolysis starts with the molecule glucose.

  • Glycolysis is the metabolic pathway that converts glucose into pyruvate.

  • Glucose is a simple sugar and the primary source of energy for cells.

  • During glycolysis, glucose is broken down into two molecules of pyruvate.

  • This process occurs in the cytoplasm of cells and is the first step in cellular respiration.

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Q5. So glucose or CO2, which is more oxidized?

Ans.

CO2 is more oxidized than glucose.

  • CO2 has a higher oxidation state than glucose.

  • In CO2, carbon is fully oxidized to its highest oxidation state (+4), while in glucose, carbon is partially oxidized to its lowest oxidation state (-4).

  • Glucose contains more reduced carbon atoms compared to CO2.

  • Glucose has more hydrogen atoms bonded to carbon, indicating a lower degree of oxidation compared to CO2.

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Q6. Different techniques to study protein-protein interaction?

Ans.

Various techniques are used to study protein-protein interactions.

  • Yeast two-hybrid assay

  • Co-immunoprecipitation

  • Surface plasmon resonance

  • Fluorescence resonance energy transfer (FRET)

  • Proximity ligation assay (PLA)

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Q7. Glucose" "and where does it finally end?

Ans.

Glucose is finally metabolized in the mitochondria to produce ATP, the energy currency of cells.

  • Glucose is broken down through a series of metabolic pathways

  • The final step of glucose metabolism occurs in the mitochondria

  • Glucose is converted into pyruvate through glycolysis

  • Pyruvate enters the mitochondria and undergoes the citric acid cycle

  • The citric acid cycle produces high-energy molecules like NADH and FADH2

  • NADH and FADH2 are used in oxidative phosphorylation to produce ATP

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Q8. Why is GFP is used so widely?

Ans.

GFP is used widely due to its unique properties and versatility.

  • GFP (Green Fluorescent Protein) is widely used as a fluorescent marker in biological research.

  • It is used to visualize and track specific proteins or cells in living organisms.

  • GFP can be genetically fused with other proteins to study their localization and dynamics.

  • It has revolutionized the field of molecular and cell biology, enabling researchers to study complex biological processes.

  • GFP is also used in medical d...read more

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Q9. Is glycolysis an oxidative process?

Ans.

Yes, glycolysis is an oxidative process.

  • Glycolysis is the metabolic pathway that converts glucose into pyruvate.

  • During glycolysis, glucose is partially oxidized to produce ATP and NADH.

  • The process involves a series of enzymatic reactions that occur in the cytoplasm of cells.

  • Although glycolysis is considered an oxidative process, it does not require oxygen.

  • The end products of glycolysis can further enter aerobic or anaerobic pathways.

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Q10. What is RFP?

Ans.

RFP stands for Request for Proposal. It is a document that organizations use to solicit proposals from potential vendors or suppliers.

  • RFP is a formal invitation for vendors to submit their proposals for a specific project or requirement.

  • It outlines the project details, requirements, evaluation criteria, and timeline.

  • RFPs are commonly used in procurement processes to ensure transparency and fair competition.

  • Vendors respond to RFPs by submitting their proposals, which are then ...read more

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Q11. What does an Analyst do?

Ans.

An Analyst is responsible for collecting, processing, and analyzing data to provide insights and recommendations for decision-making.

  • Collect and gather data from various sources

  • Process and clean data to ensure accuracy and reliability

  • Analyze data using statistical methods and tools

  • Generate reports and presentations to communicate findings

  • Provide insights and recommendations based on data analysis

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