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10+ Crown Builders Interview Questions and Answers
Q1. What is the pH of 10 to the power -8 Molar HCL?
The pH of 10^-8 Molar HCL is 8.
pH is a measure of the acidity or alkalinity of a solution.
The pH scale ranges from 0 to 14, with 7 being neutral.
A pH below 7 indicates acidity, while a pH above 7 indicates alkalinity.
The pH of a solution can be calculated using the formula pH = -log[H+].
In this case, the concentration of H+ ions is 10^-8 Molar, so the pH is 8.
Q2. Three most important reasons why DNA is the genetic material?
DNA is the genetic material due to its ability to store, replicate, and transmit genetic information.
DNA can store vast amounts of genetic information in its sequence of nucleotides.
DNA can replicate itself accurately, ensuring the transmission of genetic information to offspring.
DNA can transmit genetic information from one generation to the next through the process of inheritance.
Q3. how will you differentiate without wasting the sample much?
By using efficient testing methods and techniques, we can differentiate without wasting the sample much.
Utilize non-destructive testing methods
Implement statistical analysis to minimize sample size
Utilize advanced technology for accurate and precise measurements
Optimize testing procedures to reduce sample consumption
Q4. Which molecule does glycolysis start with?
Glycolysis starts with the molecule glucose.
Glycolysis is the metabolic pathway that converts glucose into pyruvate.
Glucose is a simple sugar and the primary source of energy for cells.
During glycolysis, glucose is broken down into two molecules of pyruvate.
This process occurs in the cytoplasm of cells and is the first step in cellular respiration.
Q5. So glucose or CO2, which is more oxidized?
CO2 is more oxidized than glucose.
CO2 has a higher oxidation state than glucose.
In CO2, carbon is fully oxidized to its highest oxidation state (+4), while in glucose, carbon is partially oxidized to its lowest oxidation state (-4).
Glucose contains more reduced carbon atoms compared to CO2.
Glucose has more hydrogen atoms bonded to carbon, indicating a lower degree of oxidation compared to CO2.
Q6. Different techniques to study protein-protein interaction?
Various techniques are used to study protein-protein interactions.
Yeast two-hybrid assay
Co-immunoprecipitation
Surface plasmon resonance
Fluorescence resonance energy transfer (FRET)
Proximity ligation assay (PLA)
Q7. Glucose" "and where does it finally end?
Glucose is finally metabolized in the mitochondria to produce ATP, the energy currency of cells.
Glucose is broken down through a series of metabolic pathways
The final step of glucose metabolism occurs in the mitochondria
Glucose is converted into pyruvate through glycolysis
Pyruvate enters the mitochondria and undergoes the citric acid cycle
The citric acid cycle produces high-energy molecules like NADH and FADH2
NADH and FADH2 are used in oxidative phosphorylation to produce ATP
Q8. Why is GFP is used so widely?
GFP is used widely due to its unique properties and versatility.
GFP (Green Fluorescent Protein) is widely used as a fluorescent marker in biological research.
It is used to visualize and track specific proteins or cells in living organisms.
GFP can be genetically fused with other proteins to study their localization and dynamics.
It has revolutionized the field of molecular and cell biology, enabling researchers to study complex biological processes.
GFP is also used in medical d...read more
Q9. Is glycolysis an oxidative process?
Yes, glycolysis is an oxidative process.
Glycolysis is the metabolic pathway that converts glucose into pyruvate.
During glycolysis, glucose is partially oxidized to produce ATP and NADH.
The process involves a series of enzymatic reactions that occur in the cytoplasm of cells.
Although glycolysis is considered an oxidative process, it does not require oxygen.
The end products of glycolysis can further enter aerobic or anaerobic pathways.
Q10. What is RFP?
RFP stands for Request for Proposal. It is a document that organizations use to solicit proposals from potential vendors or suppliers.
RFP is a formal invitation for vendors to submit their proposals for a specific project or requirement.
It outlines the project details, requirements, evaluation criteria, and timeline.
RFPs are commonly used in procurement processes to ensure transparency and fair competition.
Vendors respond to RFPs by submitting their proposals, which are then ...read more
Q11. What does an Analyst do?
An Analyst is responsible for collecting, processing, and analyzing data to provide insights and recommendations for decision-making.
Collect and gather data from various sources
Process and clean data to ensure accuracy and reliability
Analyze data using statistical methods and tools
Generate reports and presentations to communicate findings
Provide insights and recommendations based on data analysis
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