MSN Laboratories Scientific Officer Interview Questions and Answers

pKa plays a crucial role in HPLC method development by determining the ionization state of analytes and their retention on the stationary phase.

• pKa helps in selecting the appropriate mobile phase pH for ionizable compounds.

• It influences the ionization of analytes, affecting their solubility and interaction with the stationary phase.

• pKa values aid in optimizing separation by adjusting the pH to enhance selectivity and r...read more

The pKa value of a product is calculated based on its acid dissociation constant and the concentration of the acid and its conjugate base.

• pKa is a measure of the acidity or basicity of a compound.

• It is calculated using the equation: pKa = -log10(Ka), where Ka is the acid dissociation constant.

• The acid dissociation constant is the ratio of the concentration of the dissociated form of the acid to the concentration of the...read more

Titration is a technique used in chemistry to determine the concentration of a substance in a solution.

• Acid-base titration: involves the reaction between an acid and a base to determine the concentration of either

• Redox titration: involves the transfer of electrons between reactants to determine the concentration of a substance

• Complexometric titration: involves the formation of a complex between a metal ion and a ligand...read more

Developing an HPLC related substances method involves selecting appropriate column, mobile phase, and detection wavelength.

• Select a suitable column based on the analyte properties and separation requirements.

• Optimize the mobile phase composition and gradient program for efficient separation.

• Choose an appropriate detection wavelength based on the analyte's UV absorption properties.

• Validate the method by testing specific...read more

There are several types of GC capillary columns, including non-polar, polar, and specialty columns. The best type depends on the specific application and analytes being analyzed.

• Non-polar columns are best for separating non-polar compounds, such as hydrocarbons.

• Polar columns are ideal for separating polar compounds, such as alcohols and acids.

• Specialty columns, like chiral columns, are used for separating enantiomers.

• F...read more