10+ Lakhani Footwear Interview Questions and Answers

Nucleic acids are biomolecules essential for storing and transmitting genetic information in cells.

• Nucleic acids are made up of nucleotides, which consist of a sugar, a phosphate group, and a nitrogenous base.

• There are two types of nucleic acids: DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).

• DNA carries genetic information and is responsible for inheritance, while RNA plays a role in protein synthesis.

• Nucleic acids are crucial for the replication and expression of ge...read more

PCR is a technique used to amplify a specific DNA sequence, producing millions of copies for analysis.

• PCR involves cycles of heating and cooling to denature, anneal, and extend DNA strands.

• Types of PCR include conventional PCR, quantitative PCR (qPCR), reverse transcription PCR (RT-PCR), and digital PCR.

• Conventional PCR is used for DNA amplification, qPCR for quantifying DNA, RT-PCR for RNA amplification, and digital PCR for absolute quantification.

Nucleic acids are macromolecules made up of nucleotides, while nucleosides are smaller molecules composed of a nitrogenous base and a sugar.

• Nucleic acids are polymers made up of nucleotides, which consist of a nitrogenous base, a sugar, and a phosphate group.

• Examples of nucleic acids include DNA and RNA.

• Nucleosides are smaller molecules composed of a nitrogenous base and a sugar, but they lack the phosphate group found in nucleotides.

• Examples of nucleosides include adenosine ...read more

Real-Time PCR allows for monitoring the amplification of DNA in real-time, while conventional PCR requires post-amplification analysis.

• Real-Time PCR provides quantitative data in real-time, while conventional PCR only provides qualitative data after the reaction is complete.

• Real-Time PCR uses fluorescent dyes or probes to monitor amplification as it occurs, while conventional PCR relies on gel electrophoresis to visualize the final products.

• Real-Time PCR is more sensitive and...read more

DNA extraction and isolation is the process of obtaining DNA from cells for further analysis.

• Cells are lysed to release DNA

• Proteins and other contaminants are removed through precipitation or column purification

• DNA is then precipitated and washed to remove impurities

• Finally, DNA is resuspended in a buffer for storage or analysis

Purines and pyrimidines are types of nitrogenous bases found in DNA and RNA molecules.

• Purines are double-ring structures (adenine, guanine)

• Pyrimidines are single-ring structures (cytosine, thymine, uracil)

• They are essential components of nucleic acids and play a crucial role in genetic information transfer

Real-time PCR is a technique used to monitor the amplification of a targeted DNA molecule during the PCR process.

• Real-time PCR allows for the detection and quantification of specific DNA sequences in real-time as the reaction is taking place.

• It is commonly used in research, clinical diagnostics, and forensic analysis.

• Real-time PCR is more sensitive and specific than traditional PCR methods.

• Examples of applications include gene expression analysis, pathogen detection, and gene...read more

Types of Polymerase chain reaction include conventional PCR, real-time PCR, nested PCR, and reverse transcription PCR.

• Conventional PCR: amplifies a specific DNA segment using primers and a DNA polymerase enzyme

• Real-time PCR: allows for quantification of DNA during amplification process

• Nested PCR: involves two sets of primers to increase specificity

• Reverse transcription PCR: used to amplify RNA sequences by first converting them to DNA

On Load Tap Changer (OLTC) is typically installed on the high voltage side of a transformer.

• OLTC is usually installed on the high voltage side of the transformer to regulate the voltage output.

• It is important for adjusting the voltage levels based on load requirements.

• Installing OLTC on the high voltage side allows for more precise control over the output voltage.

• OLTC can be found on the primary side of the transformer in most cases.

Chemistry is the study of matter and its properties, while analytical chemistry focuses on analyzing and identifying substances.

• Chemistry is a broad field that studies the composition, structure, properties, and changes of matter.

• Analytical chemistry is a specialized branch of chemistry that focuses on the qualitative and quantitative analysis of substances.

• Chemistry involves understanding the fundamental principles of matter and its interactions, while analytical chemistry i...read more

Truenat is used for testing and diagnosing infectious diseases.

• Truenat is used in medical laboratories and healthcare facilities.

• It is used for rapid and accurate detection of infectious diseases such as tuberculosis, COVID-19, HIV, etc.

• Truenat uses molecular biology techniques to identify the presence of specific pathogens.

• It provides quick results, often within a few hours, enabling timely treatment and containment of diseases.

• Truenat is portable and can be used in remote a...read more

Chromatography is a technique used to separate and analyze mixtures of chemicals based on their properties.

• Chromatography involves a stationary phase and a mobile phase that interact with the sample mixture.

• The sample mixture is separated into its components as they move at different rates through the stationary phase.

• Different types of chromatography include gas chromatography, liquid chromatography, and thin-layer chromatography.

• Chromatography is widely used in various indu...read more