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posted on 22 Jul 2023
Peak purity is the measure of the homogeneity of a peak in a chromatogram, while % area normalization is a method to compare the relative amounts of different peaks in a chromatogram.
Peak purity is determined by comparing the spectra at different points within a peak to ensure they are similar, indicating a pure compound.
% area normalization involves adjusting the peak areas in a chromatogram to a common reference poin...
PDA detector measures absorbance at multiple wavelengths, while DAD detector measures absorbance at all wavelengths simultaneously.
PDA detector uses a photodiode array to measure absorbance at multiple wavelengths, allowing for more detailed analysis.
DAD detector uses a diode array to measure absorbance at all wavelengths simultaneously, providing faster and more comprehensive data.
PDA detector is more suitable for app...
Beer's Ambert Law states that the absorbance of a substance is directly proportional to its concentration and the path length of the light through the sample.
Beer's Ambert Law is commonly used in spectrophotometry to determine the concentration of a substance in a solution.
The law is expressed as A = εlc, where A is the absorbance, ε is the molar absorptivity, l is the path length, and c is the concentration.
It is appl...
Developing RS method development involves understanding the research objectives, selecting appropriate techniques, optimizing parameters, and validating the method.
Understand the research objectives and requirements
Select appropriate techniques based on the sample and analyte properties
Optimize method parameters such as mobile phase composition, column temperature, and flow rate
Validate the method by assessing paramete...
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