10+ Lupin Research Associate Interview Questions and Answers

I would investigate the cause and try alternative sterilization methods.

• Check if there is an issue with the equipment or the sterilization process

• Try alternative sterilization methods such as chemical sterilization or dry heat sterilization

• Consult with colleagues or experts in the field for advice

• Document the issue and any actions taken for future reference

I would choose to follow safety parameters over GLP.

• Safety of personnel and environment is of utmost importance

• GLP can be compromised temporarily but safety cannot

• Non-compliance with safety regulations can lead to serious consequences

The two most important reactions for humans, animals and plants are photosynthesis and respiration.

• Photosynthesis is the process by which plants convert sunlight, carbon dioxide and water into glucose and oxygen.

• Respiration is the process by which living organisms convert glucose and oxygen into energy, carbon dioxide and water.

• Photosynthesis and respiration are complementary processes that sustain life on Earth.

• Photosynthesis produces the oxygen that animals and humans breat...read more

Answering questions related to upstream processing, including process flow, material and system use, FIT, CIP, SIP, bioreactor system, and role of raw material in media.

• Previous job responsibilities in upstream processing

• Process flow of upstream processing

• Make and MOC of the material and system use during operation

• FIT, CIP, and SIP

• Types of and part of bioreactor system

• Role of raw material in media

Glycerol is used for long term preservation of bacterial cultures due to its ability to prevent ice crystal formation and cell damage.

• Glycerol acts as a cryoprotectant, preventing ice crystal formation and cell damage during freezing.

• It allows for long term storage of bacterial cultures at -80°C.

• Glycerol is added to the bacterial culture before freezing to a final concentration of 15-20%.

• It is a common practice in microbiology and biotechnology research.

• Other cryoprotectants ...read more

Recombinant fermentation is a process of using microorganisms to produce recombinant proteins.

• Recombinant DNA technology is used to insert a gene into a microorganism's DNA

• The microorganism is then grown in a fermentation tank to produce the desired protein

• Examples include the production of insulin using E. coli bacteria

• Optimization of fermentation conditions is crucial for high yield and purity of the protein

I have handled various types of bacterial cultures.

• I have experience with both gram-positive and gram-negative bacteria.

• I have worked with aerobic and anaerobic bacteria.

• I have handled pathogenic bacteria such as E. coli and Salmonella.

• I have also worked with non-pathogenic bacteria such as Lactobacillus and Streptococcus.

• I have experience with bacterial cultures in both liquid and solid media.

Shake flask study is a method used to evaluate the growth and productivity of microorganisms in a small-scale culture.

• It involves growing microorganisms in a small flask with shaking to provide oxygen and nutrients.

• The flask is incubated at a specific temperature and pH to mimic the conditions of the larger culture.

• Samples are taken at regular intervals to measure cell growth and product formation.

• Shake flask studies are often used in the development of bioprocesses for the p...read more

Yes, I can prepare presentations.

• I have experience creating PowerPoint presentations for research findings.

• I can effectively communicate complex data and research findings in a clear and concise manner.

• I am skilled in using visual aids and graphics to enhance the presentation.

• I am comfortable presenting to both small and large audiences.