Lupin
10+ Mikky Publication Interview Questions and Answers
Q1. What you do when Fermenters are not get sterilized with standard Sterilization protocol?
I would investigate the cause and try alternative sterilization methods.
Check if there is an issue with the equipment or the sterilization process
Try alternative sterilization methods such as chemical sterilization or dry heat sterilization
Consult with colleagues or experts in the field for advice
Document the issue and any actions taken for future reference
Q2. While working in the laboratory you realize you can only follow one of the two parameters out of Safety and GLP, which one you will choose to follow and why?
I would choose to follow safety parameters over GLP.
Safety of personnel and environment is of utmost importance
GLP can be compromised temporarily but safety cannot
Non-compliance with safety regulations can lead to serious consequences
Q3. What are the two most important reactions on which humans, animals and plants are living?
The two most important reactions for humans, animals and plants are photosynthesis and respiration.
Photosynthesis is the process by which plants convert sunlight, carbon dioxide and water into glucose and oxygen.
Respiration is the process by which living organisms convert glucose and oxygen into energy, carbon dioxide and water.
Photosynthesis and respiration are complementary processes that sustain life on Earth.
Photosynthesis produces the oxygen that animals and humans breat...read more
Q4. Previous job responsibilities. Over all process flow of upstream processing. Make and MOC of the material and system use during the operation. What is FIT,CIP and SIP. Types of and part of bioreactor system. ro...
read moreAnswering questions related to upstream processing, including process flow, material and system use, FIT, CIP, SIP, bioreactor system, and role of raw material in media.
Previous job responsibilities in upstream processing
Process flow of upstream processing
Make and MOC of the material and system use during operation
FIT, CIP, and SIP
Types of and part of bioreactor system
Role of raw material in media
Q5. Why you used glycerol for long term preservation of bacterial cultures?
Glycerol is used for long term preservation of bacterial cultures due to its ability to prevent ice crystal formation and cell damage.
Glycerol acts as a cryoprotectant, preventing ice crystal formation and cell damage during freezing.
It allows for long term storage of bacterial cultures at -80°C.
Glycerol is added to the bacterial culture before freezing to a final concentration of 15-20%.
It is a common practice in microbiology and biotechnology research.
Other cryoprotectants ...read more
Q6. What is recombinant fermentation? How you do it?
Recombinant fermentation is a process of using microorganisms to produce recombinant proteins.
Recombinant DNA technology is used to insert a gene into a microorganism's DNA
The microorganism is then grown in a fermentation tank to produce the desired protein
Examples include the production of insulin using E. coli bacteria
Optimization of fermentation conditions is crucial for high yield and purity of the protein
Q7. Which type of bacterial cultures you handled?
I have handled various types of bacterial cultures.
I have experience with both gram-positive and gram-negative bacteria.
I have worked with aerobic and anaerobic bacteria.
I have handled pathogenic bacteria such as E. coli and Salmonella.
I have also worked with non-pathogenic bacteria such as Lactobacillus and Streptococcus.
I have experience with bacterial cultures in both liquid and solid media.
Q8. Explain in detail about shake flask study.
Shake flask study is a method used to evaluate the growth and productivity of microorganisms in a small-scale culture.
It involves growing microorganisms in a small flask with shaking to provide oxygen and nutrients.
The flask is incubated at a specific temperature and pH to mimic the conditions of the larger culture.
Samples are taken at regular intervals to measure cell growth and product formation.
Shake flask studies are often used in the development of bioprocesses for the p...read more
Q9. Can you prepare presentation?
Yes, I can prepare presentations.
I have experience creating PowerPoint presentations for research findings.
I can effectively communicate complex data and research findings in a clear and concise manner.
I am skilled in using visual aids and graphics to enhance the presentation.
I am comfortable presenting to both small and large audiences.
Q10. 50 ppm solution?
A 50 ppm solution contains 50 parts of solute per million parts of solution.
ppm stands for parts per million.
It is a measure of concentration.
In a 50 ppm solution, there are 50 parts of solute per million parts of solution.
For example, if we have a 1 million mL solution, there would be 50 mL of solute in it.
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