What is principle of Hplc?

Question

Accepted Answer

HPLC stands for High Performance Liquid Chromatography. It is a technique used to separate, identify, and quantify components in a mixture.

HPLC works on the principle of differential partitioning of analytes between a stationary phase and a mobile phase.
The stationary phase is a solid or liquid material packed into a column, while the mobile phase is a liquid solvent that carries the sample through the column.
Analytes with different affinities for the stationary phase and mobile phase will separate based on their interactions.
Retention time is the time taken for an analyte to pass through the column, and it helps in identification and quantification.
Different types of HPLC techniques include reverse phase, normal phase, ion exchange, size exclusion, and affinity chromatography.
HPLC is widely used in various fields such as pharmaceuticals, environmental analysis, forensics, and food testing.

Accepted Answer

The non volatile substance separation of mixture of compound in that stationary phase is solid and mobile phase is liquid of

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