Anjan Drug
10+ AnandKrupa Publicity Interview Questions and Answers
Q1. What is pH meter , How to calibrate pH meter
A pH meter is a scientific instrument used to measure the acidity or alkalinity of a substance. Calibration ensures accurate readings.
pH meter measures the concentration of hydrogen ions in a solution
Calibration involves adjusting the pH meter to known standard solutions
Regular calibration ensures accurate readings over time
Q2. What is Titration, What is pH
Titration is a technique to determine the concentration of a substance in a solution. pH is a measure of acidity or basicity of a solution.
Titration involves adding a solution of known concentration to a solution of unknown concentration until the reaction is complete.
pH is measured on a scale of 0-14, with 7 being neutral, below 7 acidic, and above 7 basic.
Titration can be used to determine the concentration of acids, bases, and other substances in a solution.
pH is important...read more
Q3. PH level example, what is ph?
pH is a measure of acidity or alkalinity of a solution on a scale of 0 to 14.
pH stands for 'potential of Hydrogen'
It measures the concentration of hydrogen ions in a solution
A pH of 7 is considered neutral, below 7 is acidic, and above 7 is alkaline
Examples: Lemon juice has a pH of around 2, pure water has a pH of 7, and baking soda has a pH of around 9
Q4. What are the buffer used in HPLC
Buffers commonly used in HPLC include phosphate buffers, acetate buffers, and citrate buffers.
Phosphate buffers are commonly used in HPLC for separating acidic compounds.
Acetate buffers are used for separating basic compounds.
Citrate buffers are used for separating metal ions.
Other common buffers used in HPLC include ammonium acetate and ammonium formate.
Q5. Any Nameing reaction Mechanism
The Grignard reaction involves the addition of an organomagnesium halide to a carbonyl group.
The reaction is named after French chemist Victor Grignard.
It is used to form carbon-carbon bonds and is a key reaction in organic synthesis.
An example is the synthesis of triphenylmethanol from benzophenone and phenylmagnesium bromide.
The reaction proceeds through the formation of a Grignard reagent, which then attacks the carbonyl group.
The resulting intermediate then undergoes hydr...read more
Q6. Fuel description
Fuel is a substance that is burned to produce energy.
Fuel can be in the form of gas, liquid, or solid.
Examples of fuels include gasoline, diesel, natural gas, coal, and wood.
The type of fuel used depends on the application and availability.
Fuels are used in various industries such as transportation, power generation, and heating.
Efficient use of fuel is important to reduce environmental impact and cost.
Q7. Why propanol has high boiling point than ethanol
Propanol has a higher boiling point than ethanol due to stronger intermolecular forces.
Propanol has a longer carbon chain than ethanol, leading to increased surface area for intermolecular interactions.
Propanol molecules have more Van der Waals forces compared to ethanol.
Hydrogen bonding in propanol is stronger than in ethanol, contributing to higher boiling point.
Q8. How to calibrate HPLC
HPLC calibration involves adjusting parameters to ensure accurate and reliable results.
Prepare standard solutions of known concentrations
Run the standard solutions through the HPLC system
Compare the results to expected values and adjust parameters as needed
Repeat the process until accurate and consistent results are obtained
Q9. What is called as clemenson reduction
Clemmensen reduction is a chemical reaction that involves the reduction of a carbonyl group to a methylene group using zinc amalgam and hydrochloric acid.
Clemmensen reduction is a useful method for converting ketones and aldehydes into alkanes.
The reaction is carried out under acidic conditions with zinc amalgam as the reducing agent.
The mechanism involves the formation of a complex between the carbonyl group and zinc, followed by protonation and reduction steps.
Clemmensen re...read more
Q10. Why use caffeine for calibration of HPLC
Caffeine is used for calibration of HPLC due to its well-defined retention time and peak shape.
Caffeine has a well-defined retention time, making it easy to identify and calibrate the HPLC system.
Caffeine produces a sharp and symmetrical peak, which helps in accurately determining the system's performance.
It is a commonly used compound for calibration in HPLC due to its stability and reproducibility.
Using caffeine as a calibration standard allows for consistent and reliable r...read more
Q11. Explain the parts of HPLC
HPLC stands for High Performance Liquid Chromatography, which consists of a pump, injector, column, detector, and integrator.
HPLC involves a pump to push the liquid solvent through the system.
It includes an injector to introduce the sample into the mobile phase.
The column separates the components of the sample based on their interactions with the stationary phase.
A detector measures the amount of analyte in the eluent.
An integrator records and analyzes the data from the detec...read more
Q12. how to solve customer requirements ?
Customer requirements can be solved by understanding their needs, communicating effectively, and delivering high-quality products or services.
Listen carefully to the customer's needs and expectations
Communicate clearly and regularly with the customer to ensure alignment
Develop a detailed plan to meet the customer's requirements
Ensure quality control measures are in place to deliver a high-quality product or service
Seek feedback from the customer to make necessary adjustments
Q13. Trouble shooting of HPLC
Troubleshooting of HPLC involves identifying and resolving issues with the high-performance liquid chromatography system.
Check for leaks in the system and tighten connections if necessary.
Inspect and clean the columns regularly to ensure optimal performance.
Calibrate the detector and pump to maintain accuracy of results.
Troubleshoot error messages on the HPLC software by checking system settings and parameters.
Consult the user manual or contact technical support for further a...read more
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