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10+ AnandKrupa Publicity Interview Questions and Answers

Updated 21 Jun 2024

Q1. What is pH meter , How to calibrate pH meter

Ans.

A pH meter is a scientific instrument used to measure the acidity or alkalinity of a substance. Calibration ensures accurate readings.

  • pH meter measures the concentration of hydrogen ions in a solution

  • Calibration involves adjusting the pH meter to known standard solutions

  • Regular calibration ensures accurate readings over time

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Q2. What is Titration, What is pH

Ans.

Titration is a technique to determine the concentration of a substance in a solution. pH is a measure of acidity or basicity of a solution.

  • Titration involves adding a solution of known concentration to a solution of unknown concentration until the reaction is complete.

  • pH is measured on a scale of 0-14, with 7 being neutral, below 7 acidic, and above 7 basic.

  • Titration can be used to determine the concentration of acids, bases, and other substances in a solution.

  • pH is important...read more

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Q3. PH level example, what is ph?

Ans.

pH is a measure of acidity or alkalinity of a solution on a scale of 0 to 14.

  • pH stands for 'potential of Hydrogen'

  • It measures the concentration of hydrogen ions in a solution

  • A pH of 7 is considered neutral, below 7 is acidic, and above 7 is alkaline

  • Examples: Lemon juice has a pH of around 2, pure water has a pH of 7, and baking soda has a pH of around 9

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Q4. What are the buffer used in HPLC

Ans.

Buffers commonly used in HPLC include phosphate buffers, acetate buffers, and citrate buffers.

  • Phosphate buffers are commonly used in HPLC for separating acidic compounds.

  • Acetate buffers are used for separating basic compounds.

  • Citrate buffers are used for separating metal ions.

  • Other common buffers used in HPLC include ammonium acetate and ammonium formate.

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Q5. Any Nameing reaction Mechanism

Ans.

The Grignard reaction involves the addition of an organomagnesium halide to a carbonyl group.

  • The reaction is named after French chemist Victor Grignard.

  • It is used to form carbon-carbon bonds and is a key reaction in organic synthesis.

  • An example is the synthesis of triphenylmethanol from benzophenone and phenylmagnesium bromide.

  • The reaction proceeds through the formation of a Grignard reagent, which then attacks the carbonyl group.

  • The resulting intermediate then undergoes hydr...read more

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Q6. Fuel description

Ans.

Fuel is a substance that is burned to produce energy.

  • Fuel can be in the form of gas, liquid, or solid.

  • Examples of fuels include gasoline, diesel, natural gas, coal, and wood.

  • The type of fuel used depends on the application and availability.

  • Fuels are used in various industries such as transportation, power generation, and heating.

  • Efficient use of fuel is important to reduce environmental impact and cost.

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Q7. Why propanol has high boiling point than ethanol

Ans.

Propanol has a higher boiling point than ethanol due to stronger intermolecular forces.

  • Propanol has a longer carbon chain than ethanol, leading to increased surface area for intermolecular interactions.

  • Propanol molecules have more Van der Waals forces compared to ethanol.

  • Hydrogen bonding in propanol is stronger than in ethanol, contributing to higher boiling point.

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Q8. How to calibrate HPLC

Ans.

HPLC calibration involves adjusting parameters to ensure accurate and reliable results.

  • Prepare standard solutions of known concentrations

  • Run the standard solutions through the HPLC system

  • Compare the results to expected values and adjust parameters as needed

  • Repeat the process until accurate and consistent results are obtained

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Q9. What is called as clemenson reduction

Ans.

Clemmensen reduction is a chemical reaction that involves the reduction of a carbonyl group to a methylene group using zinc amalgam and hydrochloric acid.

  • Clemmensen reduction is a useful method for converting ketones and aldehydes into alkanes.

  • The reaction is carried out under acidic conditions with zinc amalgam as the reducing agent.

  • The mechanism involves the formation of a complex between the carbonyl group and zinc, followed by protonation and reduction steps.

  • Clemmensen re...read more

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Q10. Why use caffeine for calibration of HPLC

Ans.

Caffeine is used for calibration of HPLC due to its well-defined retention time and peak shape.

  • Caffeine has a well-defined retention time, making it easy to identify and calibrate the HPLC system.

  • Caffeine produces a sharp and symmetrical peak, which helps in accurately determining the system's performance.

  • It is a commonly used compound for calibration in HPLC due to its stability and reproducibility.

  • Using caffeine as a calibration standard allows for consistent and reliable r...read more

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Q11. Explain the parts of HPLC

Ans.

HPLC stands for High Performance Liquid Chromatography, which consists of a pump, injector, column, detector, and integrator.

  • HPLC involves a pump to push the liquid solvent through the system.

  • It includes an injector to introduce the sample into the mobile phase.

  • The column separates the components of the sample based on their interactions with the stationary phase.

  • A detector measures the amount of analyte in the eluent.

  • An integrator records and analyzes the data from the detec...read more

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Q12. how to solve customer requirements ?

Ans.

Customer requirements can be solved by understanding their needs, communicating effectively, and delivering high-quality products or services.

  • Listen carefully to the customer's needs and expectations

  • Communicate clearly and regularly with the customer to ensure alignment

  • Develop a detailed plan to meet the customer's requirements

  • Ensure quality control measures are in place to deliver a high-quality product or service

  • Seek feedback from the customer to make necessary adjustments

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Q13. Trouble shooting of HPLC

Ans.

Troubleshooting of HPLC involves identifying and resolving issues with the high-performance liquid chromatography system.

  • Check for leaks in the system and tighten connections if necessary.

  • Inspect and clean the columns regularly to ensure optimal performance.

  • Calibrate the detector and pump to maintain accuracy of results.

  • Troubleshoot error messages on the HPLC software by checking system settings and parameters.

  • Consult the user manual or contact technical support for further a...read more

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