Junior Embryologist

Junior Embryologist Interview Questions and Answers

Updated 24 Sep 2024

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Q1. What is IVF, and how does it differ from natural conception?

Ans.

IVF is a fertility treatment where eggs and sperm are combined outside the body, unlike natural conception.

  • IVF stands for in vitro fertilization

  • In IVF, eggs are retrieved from the woman's ovaries and fertilized with sperm in a lab dish

  • The resulting embryos are then transferred back into the woman's uterus for implantation

  • IVF is used to treat infertility or genetic disorders, and can involve the use of donor eggs or sperm

  • Natural conception occurs when sperm fertilizes an egg i...read more

Q2. What is IUI, and how does it work?In what situations is IUI recommended?

Ans.

IUI stands for intrauterine insemination, a fertility treatment where sperm is directly placed in the uterus to increase the chances of fertilization.

  • IUI involves washing and preparing sperm before inserting it into the uterus.

  • It is recommended for couples with unexplained infertility, mild male factor infertility, or cervical mucus issues.

  • IUI can also be used for single women or same-sex couples using donor sperm.

  • Success rates of IUI vary depending on the underlying cause of...read more

Junior Embryologist Interview Questions and Answers for Freshers

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Q3. What are the main steps involved in the IVF process?

Ans.

The main steps in the IVF process involve ovarian stimulation, egg retrieval, fertilization, embryo culture, and embryo transfer.

  • Ovarian stimulation: Hormonal medications are used to stimulate the ovaries to produce multiple eggs.

  • Egg retrieval: The eggs are retrieved from the ovaries using a needle guided by ultrasound.

  • Fertilization: The eggs are fertilized with sperm in a laboratory dish to create embryos.

  • Embryo culture: The embryos are cultured in a special incubator for a ...read more

Q4. What are the steps involved in the IUI procedure?

Ans.

IUI procedure involves preparing sperm, inserting it into the uterus using a catheter, and monitoring for pregnancy.

  • Sperm preparation: Semen sample is collected and washed to separate sperm from other components.

  • Catheter insertion: A thin tube is used to insert prepared sperm into the uterus.

  • Monitoring: Patient is monitored for signs of pregnancy after the procedure.

  • Ovulation induction: In some cases, medications may be used to stimulate ovulation before IUI.

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Q5. What happens to the embryos after fertilization?

Ans.

After fertilization, embryos undergo cleavage, blastulation, implantation, and eventually develop into a fetus.

  • Embryos undergo cleavage, where they divide into smaller cells called blastomeres.

  • Blastulation follows, forming a blastocyst with an inner cell mass and outer trophoblast.

  • The blastocyst implants into the uterine wall during implantation.

  • The embryo continues to develop into a fetus, undergoing various stages of growth and differentiation.

Q6. How is the specimen prepared for IUI?

Ans.

Specimen for IUI is prepared by washing and processing the sperm to remove impurities and increase motility.

  • Sperm sample is collected and washed to remove seminal fluid, debris, and dead sperm.

  • The sperm is then processed to separate motile sperm from immotile sperm and other impurities.

  • The processed sperm is then loaded into a catheter for insertion into the uterus during the IUI procedure.

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Q7. What is the most important thing to do in ivf lab

Ans.

Maintaining strict quality control and adhering to protocols are the most important things to do in an IVF lab.

  • Maintain strict quality control to ensure accurate results and successful outcomes.

  • Adhere to protocols and guidelines set by regulatory bodies to maintain ethical standards.

  • Regularly calibrate and maintain equipment to ensure accuracy and reliability of results.

  • Properly document all procedures and results for traceability and quality assurance.

  • Ensure a clean and ster...read more

Q8. How to work in all circumstances

Ans.

To work in all circumstances, one must be adaptable, flexible, and have problem-solving skills.

  • Be open to change and willing to learn new things

  • Stay calm and focused under pressure

  • Think creatively to find solutions to unexpected challenges

  • Communicate effectively with colleagues and superiors

  • Be organized and prioritize tasks based on importance

  • Maintain a positive attitude and be a team player

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Q9. What are the methods of sterilization

Ans.

Methods of sterilization include heat, chemicals, radiation, and filtration.

  • Heat sterilization: Autoclaving, dry heat sterilization

  • Chemical sterilization: Ethylene oxide, hydrogen peroxide

  • Radiation sterilization: Gamma radiation, electron beam

  • Filtration sterilization: Membrane filtration, depth filtration

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