Junior Embryologist

Junior Embryologist Interview Questions and Answers for Freshers

Updated 12 Jul 2025

Q. What is IVF, and how does it differ from natural conception?

Ans.

IVF is a fertility treatment where eggs and sperm are combined outside the body, unlike natural conception.

  • IVF stands for in vitro fertilization

  • In IVF, eggs are retrieved from the woman's ovaries and fertilized with sperm in a lab dish

  • The resulting embryos are then transferred back into the woman's uterus for implantation

  • IVF is used to treat infertility or genetic disorders, and can involve the use of donor eggs or sperm

  • Natural conception occurs when sperm fertilizes an egg i...read more

Q. What is IUI, and how does it work?In what situations is IUI recommended?

Ans.

IUI stands for intrauterine insemination, a fertility treatment where sperm is directly placed in the uterus to increase the chances of fertilization.

  • IUI involves washing and preparing sperm before inserting it into the uterus.

  • It is recommended for couples with unexplained infertility, mild male factor infertility, or cervical mucus issues.

  • IUI can also be used for single women or same-sex couples using donor sperm.

  • Success rates of IUI vary depending on the underlying cause of...read more

Q. What are the main steps involved in the IVF process?

Ans.

The main steps in the IVF process involve ovarian stimulation, egg retrieval, fertilization, embryo culture, and embryo transfer.

  • Ovarian stimulation: Hormonal medications are used to stimulate the ovaries to produce multiple eggs.

  • Egg retrieval: The eggs are retrieved from the ovaries using a needle guided by ultrasound.

  • Fertilization: The eggs are fertilized with sperm in a laboratory dish to create embryos.

  • Embryo culture: The embryos are cultured in a special incubator for a ...read more

Q. What are the steps involved in the IUI procedure?

Ans.

IUI procedure involves preparing sperm, inserting it into the uterus using a catheter, and monitoring for pregnancy.

  • Sperm preparation: Semen sample is collected and washed to separate sperm from other components.

  • Catheter insertion: A thin tube is used to insert prepared sperm into the uterus.

  • Monitoring: Patient is monitored for signs of pregnancy after the procedure.

  • Ovulation induction: In some cases, medications may be used to stimulate ovulation before IUI.

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Q. What happens to the embryos after fertilization?

Ans.

After fertilization, embryos undergo cleavage, blastulation, implantation, and eventually develop into a fetus.

  • Embryos undergo cleavage, where they divide into smaller cells called blastomeres.

  • Blastulation follows, forming a blastocyst with an inner cell mass and outer trophoblast.

  • The blastocyst implants into the uterine wall during implantation.

  • The embryo continues to develop into a fetus, undergoing various stages of growth and differentiation.

Q. How is the specimen prepared for IUI?

Ans.

Specimen for IUI is prepared by washing and processing the sperm to remove impurities and increase motility.

  • Sperm sample is collected and washed to remove seminal fluid, debris, and dead sperm.

  • The sperm is then processed to separate motile sperm from immotile sperm and other impurities.

  • The processed sperm is then loaded into a catheter for insertion into the uterus during the IUI procedure.

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