Vital Laboratories Production Chemist Interview Questions and Answers

Q1. Naming reactions
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HPLc,GC, IR,UV,And basic chemistry related questions

Q1. What is pH meter , How to calibrate pH meter
Ans. 
A pH meter is a scientific instrument used to measure the acidity or alkalinity of a substance. Calibration ensures accurate readings.
• pH meter measures the concentration of hydrogen ions in a solution

• Calibration involves adjusting the pH meter to known standard solutions

• Regular calibration ensures accurate readings over time
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Q2. Explain the parts of HPLC
Ans. 
HPLC stands for High Performance Liquid Chromatography, which consists of a pump, injector, column, detector, and integrator.
• HPLC involves a pump to push the liquid solvent through the system.

• It includes an injector to introduce the sample into the mobile phase.

• The column separates the components of the sample based on their interactions with the stationary phase.

• A detector measures the amount of analyte in the eluent.

• ...read more
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Q1. What are the buffer used in HPLC
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Buffers commonly used in HPLC include phosphate buffers, acetate buffers, and citrate buffers.
• Phosphate buffers are commonly used in HPLC for separating acidic compounds.

• Acetate buffers are used for separating basic compounds.

• Citrate buffers are used for separating metal ions.

• Other common buffers used in HPLC include ammonium acetate and ammonium formate.
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Q2. How to calibrate HPLC
Ans. 
HPLC calibration involves adjusting parameters to ensure accurate and reliable results.
• Prepare standard solutions of known concentrations

• Run the standard solutions through the HPLC system

• Compare the results to expected values and adjust parameters as needed

• Repeat the process until accurate and consistent results are obtained
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Total 30 Questions (15 Aptitude and 15 Technical Questions)

Q1. Any Nameing reaction Mechanism
Ans. 
The Grignard reaction involves the addition of an organomagnesium halide to a carbonyl group.
• The reaction is named after French chemist Victor Grignard.

• It is used to form carbon-carbon bonds and is a key reaction in organic synthesis.

• An example is the synthesis of triphenylmethanol from benzophenone and phenylmagnesium bromide.

• The reaction proceeds through the formation of a Grignard reagent, which then attacks the car...read more
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Q2. What is Titration, What is pH
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Titration is a technique to determine the concentration of a substance in a solution. pH is a measure of acidity or basicity of a solution.
• Titration involves adding a solution of known concentration to a solution of unknown concentration until the reaction is complete.

• pH is measured on a scale of 0-14, with 7 being neutral, below 7 acidic, and above 7 basic.

• Titration can be used to determine the concentration of acids,...read more
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Q1. Waiting For 2 Months
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Q1. How to solve customer requirements ?
Ans. 
Customer requirements can be solved by understanding their needs, communicating effectively, and delivering high-quality products or services.
• Listen carefully to the customer's needs and expectations

• Communicate clearly and regularly with the customer to ensure alignment

• Develop a detailed plan to meet the customer's requirements

• Ensure quality control measures are in place to deliver a high-quality product or service

• Seek...read more
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