Vasudha Pharma Chem Senior Executive Interview Questions and Answers

The startup procedure of HPLC involves several steps to ensure proper functioning and accurate results.

• Check the power supply and ensure the instrument is properly connected.

• Turn on the HPLC system and allow it to warm up for a specified period.

• Check the mobile phase and ensure it is prepared correctly.

• Prime the pump by purging air bubbles from the system.

• Load the sample onto the injection port or autosampler.

• Set the d...read more

Water content mechanism can be found through various methods and is important for various reasons.

• Water content can be measured through techniques such as gravimetric analysis, Karl Fischer titration, and moisture analyzers.

• Water content is important in industries such as food, pharmaceuticals, and cosmetics to ensure product quality and stability.

• Water content is also important in environmental studies to understand t...read more

Developing HPLC methods involves selecting appropriate columns and optimizing parameters.

• Choose a suitable column based on the analyte's properties and the separation goal.

• Optimize parameters such as mobile phase composition, flow rate, and temperature.

• Validate the method by assessing its selectivity, sensitivity, accuracy, and precision.

• Common column types include C18, C8, and phenyl.

• Other factors to consider include ...read more

Carbon loading in column refers to the amount of carbon used in the stationary phase of a chromatography column. Endcapping is a process to block the remaining silanol groups on the surface of the stationary phase.

• Carbon loading is typically expressed as a percentage of the total weight of the stationary phase.

• Higher carbon loading can improve the selectivity and retention of certain analytes.

• Endcapping is important to...read more