V S International Executive Interview Questions and Answers for Experienced

Q1. What is pH meter , How to calibrate pH meter
Ans. 
A pH meter is a scientific instrument used to measure the acidity or alkalinity of a substance. Calibration ensures accurate readings.
• pH meter measures the concentration of hydrogen ions in a solution

• Calibration involves adjusting the pH meter to known standard solutions

• Regular calibration ensures accurate readings over time
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Q2. Explain the parts of HPLC
Ans. 
HPLC stands for High Performance Liquid Chromatography, which consists of a pump, injector, column, detector, and integrator.
• HPLC involves a pump to push the liquid solvent through the system.

• It includes an injector to introduce the sample into the mobile phase.

• The column separates the components of the sample based on their interactions with the stationary phase.

• A detector measures the amount of analyte in the eluent.

• ...read more
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Q1. What are the buffer used in HPLC
Ans. 
Buffers commonly used in HPLC include phosphate buffers, acetate buffers, and citrate buffers.
• Phosphate buffers are commonly used in HPLC for separating acidic compounds.

• Acetate buffers are used for separating basic compounds.

• Citrate buffers are used for separating metal ions.

• Other common buffers used in HPLC include ammonium acetate and ammonium formate.
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Q2. How to calibrate HPLC
Ans. 
HPLC calibration involves adjusting parameters to ensure accurate and reliable results.
• Prepare standard solutions of known concentrations

• Run the standard solutions through the HPLC system

• Compare the results to expected values and adjust parameters as needed

• Repeat the process until accurate and consistent results are obtained
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Q1. Type of distillation column
Ans. 
Distillation columns are classified based on their internal structure and operation.
• Distillation columns can be classified as batch or continuous columns.

• Common types of distillation columns include simple distillation columns, fractional distillation columns, and steam distillation columns.

• The type of distillation column used depends on the specific separation requirements and properties of the components being separa
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Q2. Solvent boiling point
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Q3. Flash point,fire point
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Q1. Chemical Name and formula
Ans. 
Chemical name and formula are identifiers for specific chemical compounds.
• Chemical name is the common name given to a chemical compound.

• Chemical formula represents the elements and their ratios in a compound.

• Example: Water - Chemical name, H2O - Chemical formula.
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Q2. How to make 0.1M HCL
Ans. 
To make 0.1M HCL, dilute concentrated hydrochloric acid with water.
• Dilute 1 part of concentrated HCL with 9 parts of water to make 0.1M HCL solution.

• Always add acid to water slowly to prevent splashing and heat generation.

• Use proper protective equipment such as gloves and goggles when handling acids.
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Q1. Which is RF Volue and what is TLC
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Q2. Tin layer chromatography Product trafared distence/Mobile phase Trafared distence
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Q3. Purification and recrestlation
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Q1. Trouble shooting of HPLC
Ans. 
Troubleshooting of HPLC involves identifying and resolving issues with the high-performance liquid chromatography system.
• Check for leaks in the system and tighten connections if necessary.

• Inspect and clean the columns regularly to ensure optimal performance.

• Calibrate the detector and pump to maintain accuracy of results.

• Troubleshoot error messages on the HPLC software by checking system settings and parameters.

• Consult ...read more
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Q2. Why use caffeine for calibration of HPLC
Ans. 
Caffeine is used for calibration of HPLC due to its well-defined retention time and peak shape.
• Caffeine has a well-defined retention time, making it easy to identify and calibrate the HPLC system.

• Caffeine produces a sharp and symmetrical peak, which helps in accurately determining the system's performance.

• It is a commonly used compound for calibration in HPLC due to its stability and reproducibility.

• Using caffeine as a...read more
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Q1. Topics related to Compressor, Pump, Boiler
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