SynZeal Research Research Associate Interview Questions and Answers

Mass spectrometry is a technique used to identify and quantify molecules based on their mass-to-charge ratio.

• Electrospray ionization (ESI)

• Matrix-assisted laser desorption/ionization (MALDI)

• Gas chromatography-mass spectrometry (GC-MS)

• Liquid chromatography-mass spectrometry (LC-MS)

• Time-of-flight mass spectrometry (TOF-MS)

UV spectrometry is a technique that measures the absorption or transmission of ultraviolet light by a sample.

• UV spectrometry is based on the principle that different molecules absorb UV light at specific wavelengths.

• It is commonly used in analytical chemistry to determine the concentration of a substance in a sample.

• UV spectrometry can be used to analyze the purity of a compound or identify unknown substances.

• The instr...read more

To prepare 0.1 N HCl and NaOH, you need to calculate the required amount of the respective chemicals and dilute them in water.

• Calculate the molar mass of HCl and NaOH

• Determine the volume of solution needed

• Use the formula: Normality (N) = (Number of equivalents / Volume of solution in liters)

• For 0.1 N HCl, calculate the number of equivalents of HCl and dilute it to the desired volume

• For NaOH, calculate the number of equ...read more

Quality assurance is the process of ensuring that products or services meet the desired level of quality.

• It involves establishing standards and procedures to ensure that products or services meet those standards.

• It includes monitoring and evaluating the quality of products or services to identify areas for improvement.

• It also involves taking corrective action when necessary to address quality issues.

• Examples include co...read more

Recall definitions

• Recall: the ability to retrieve information from memory

• Definition: a statement that explains the meaning of a word or phrase

• Research: the systematic investigation into and study of materials and sources in order to establish facts and reach new conclusions

• Associate: a person who works with others towards a common goal

• Hypothesis: a proposed explanation for a phenomenon

• Experiment: a scientific procedure...read more

The type of HPLC used depends on the specific research requirements and objectives.

• There are several types of HPLC systems available, including reverse phase, normal phase, ion exchange, size exclusion, and affinity chromatography.

• The choice of HPLC system depends on the properties of the analyte, such as polarity, size, charge, and affinity.

• For example, reverse phase HPLC is commonly used for the separation of nonpola...read more

UV detectors and HPLC detectors are used in research to analyze and quantify substances.

• UV detectors are commonly used in UV-visible spectroscopy to measure the absorption of light by a sample at different wavelengths.

• HPLC detectors are used in high-performance liquid chromatography to detect and quantify the components of a mixture.

• Common types of UV detectors include diode array detectors (DAD) and photodiode array d...read more

Matrix in MALDI MS system is a substance used to assist in ionization of the sample for analysis.

• Matrix is a substance that absorbs energy from the laser and transfers it to the sample, causing ionization.

• The choice of matrix depends on the type of sample being analyzed.

• Common matrices include alpha-cyano-4-hydroxycinnamic acid (CHCA) and sinapinic acid (SA).

I would investigate the cause and try alternative sterilization methods.

• Check if there is an issue with the equipment or the sterilization process

• Try alternative sterilization methods such as chemical sterilization or dry heat sterilization

• Consult with colleagues or experts in the field for advice

• Document the issue and any actions taken for future reference

Shake flask study is a method used to evaluate the growth and productivity of microorganisms in a small-scale culture.

• It involves growing microorganisms in a small flask with shaking to provide oxygen and nutrients.

• The flask is incubated at a specific temperature and pH to mimic the conditions of the larger culture.

• Samples are taken at regular intervals to measure cell growth and product formation.

• Shake flask studies a...read more

Glycerol is used for long term preservation of bacterial cultures due to its ability to prevent ice crystal formation and cell damage.

• Glycerol acts as a cryoprotectant, preventing ice crystal formation and cell damage during freezing.

• It allows for long term storage of bacterial cultures at -80°C.

• Glycerol is added to the bacterial culture before freezing to a final concentration of 15-20%.

• It is a common practice in micr...read more

I would choose to follow safety parameters over GLP.

• Safety of personnel and environment is of utmost importance

• GLP can be compromised temporarily but safety cannot

• Non-compliance with safety regulations can lead to serious consequences

The two most important reactions for humans, animals and plants are photosynthesis and respiration.

• Photosynthesis is the process by which plants convert sunlight, carbon dioxide and water into glucose and oxygen.

• Respiration is the process by which living organisms convert glucose and oxygen into energy, carbon dioxide and water.

• Photosynthesis and respiration are complementary processes that sustain life on Earth.

• Photos...read more

My 12th marks were affected due to personal reasons, but I have consistently performed well in other academic pursuits.

• I faced some personal challenges during my 12th grade which affected my performance.

• However, I have consistently performed well in other academic pursuits such as my graduation.

• My 10th grade marks are a reflection of my academic potential and I have worked hard to maintain that level of performance.

• I h...read more

Answering questions related to upstream processing, including process flow, material and system use, FIT, CIP, SIP, bioreactor system, and role of raw material in media.

• Previous job responsibilities in upstream processing

• Process flow of upstream processing

• Make and MOC of the material and system use during operation

• FIT, CIP, and SIP

• Types of and part of bioreactor system

• Role of raw material in media