What is principle of HPLC???

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HPLC stands for High Performance Liquid Chromatography. It is a technique used to separate, identify, and quantify components in a mixture.

HPLC uses a stationary phase and a mobile phase to separate components based on their physical and chemical properties.
The stationary phase is typically a solid or liquid packed into a column, while the mobile phase is a liquid or gas that flows through the column.
Components in the mixture interact differently with the stationary and mobile phases, causing them to separate and elute from the column at different times.
HPLC is commonly used in pharmaceuticals, food and beverage, environmental analysis, and forensic science.
Different types of HPLC include reverse-phase, normal-phase, ion-exchange, and size-exclusion chromatography.

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