Principle of HPLC and working principle?

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HPLC is a chromatographic technique used to separate, identify, and quantify components in a mixture based on their interactions with a stationary phase and a mobile phase.

HPLC stands for High Performance Liquid Chromatography.
It involves passing a liquid sample through a column packed with a stationary phase, where different components interact differently and are separated based on their affinity for the stationary phase.
The separated components are then detected and quantified using a detector, such as UV-Vis spectroscopy or mass spectrometry.
HPLC is widely used in pharmaceutical, environmental, food, and forensic analysis for its high sensitivity and ability to separate complex mixtures.
The working principle of HPLC is based on the differential partitioning of analytes between the stationary and mobile phases, leading to their separation and detection.

Principle of HPLC and working principle?

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