How to check absorbance in UV visible spectrophotometer

Question

Accepted Answer

To check absorbance in a UV visible spectrophotometer, prepare a sample, set the wavelength, adjust the blank, and measure the absorbance.

Prepare a sample by diluting or extracting the substance of interest.
Set the wavelength on the spectrophotometer to the desired value.
Adjust the blank by measuring the absorbance of a solvent or reagent without the substance.
Measure the absorbance of the sample by placing it in the spectrophotometer and recording the reading.
Repeat the process for multiple samples or wavelengths as required.

Accepted Answer

First we set Perticular wavelength, Then stable the line with blank, Do a zero absorbance,then finally what we want to check the absorbance check Perticular wavelength.

How to check absorbance in UV visible spectrophotometer

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