10+ Interview Questions and Answers

Column volume and bed height affect protein binding differently. Column selection for protein separation depends on various factors.

• Column volume refers to the total volume of the column, while bed height is the height of the resin bed inside the column.

• Protein binding is influenced by the surface area available for binding in the column, which is directly related to bed height.

• A larger column volume allows for more protein binding capacity, while a taller bed height increase...read more

I chose this organization and biopharma due to their strong experience, stability, and alignment with my career goals.

• I chose this organization because of their extensive experience in the biopharma industry.

• The organization has a proven track record of stability and success.

• I believe that working in the biopharma field will allow me to make a meaningful impact on patients' lives.

• The organization's mission and values align with my own career goals and aspirations.

• I am impress...read more

Change controls and deviations investigation procedures involve risk assessment and escalations.

• Establish clear change control procedures to document and track any changes made to processes or systems.

• Implement a deviations investigation procedure to identify, assess, and address any deviations from standard practices.

• Conduct risk assessments to evaluate the potential impact of changes or deviations on operations and quality.

• Escalate significant changes or deviations to highe...read more

Proteins are macromolecules composed of amino acids. Aromatic amino acids are a type of amino acid with a specific chemical structure.

• Proteins are essential for the structure, function, and regulation of the body's tissues and organs.

• There are 20 different types of amino acids that make up proteins.

• Amino acids are classified into three groups: essential, nonessential, and conditional.

• Aromatic amino acids have a ring structure in their chemical composition.

• The three aromatic a...read more

GxP issues and procedures refer to Good Practice standards in regulated industries to ensure compliance with regulatory requirements.

• GxP issues include Good Manufacturing Practice (GMP), Good Laboratory Practice (GLP), and Good Clinical Practice (GCP).

• Procedures must be documented, followed consistently, and regularly reviewed for compliance.

• Regular training and audits are essential to ensure adherence to GxP standards.

• Examples of GxP procedures include maintaining accurate r...read more

The type of water produced by the MCDP is potable water.

• Potable water is safe for drinking and cooking.

• It meets the standards set by regulatory agencies for human consumption.

• Examples of sources of potable water include treated tap water and bottled water.

DNA and RNA are genetic materials. Molarity and Normality are units of concentration. 1 molar solution is prepared by dissolving 1 mole of solute in 1 liter of solvent.

• DNA and RNA are nucleic acids that carry genetic information

• Molarity is the number of moles of solute per liter of solution

• Normality is the number of equivalents of solute per liter of solution

• To prepare 1 molar solution, dissolve 1 mole of solute in 1 liter of solvent

• For example, to prepare 1 molar solution of...read more

Neutralization is a chemical reaction between an acid and a base to form a neutral solution.

• Neutralization involves the combination of H+ ions from an acid and OH- ions from a base to form water and a salt.

• It is important in various industries such as pharmaceuticals, food and beverage, and wastewater treatment.

• Neutralization helps in controlling pH levels, preventing corrosion, and ensuring product quality.

• Example: Mixing hydrochloric acid (HCl) with sodium hydroxide (NaOH) ...read more

Global regulatory aspect refers to the laws and regulations that govern businesses on an international scale.

• Understanding and complying with regulations in different countries

• Adapting to changes in regulations across borders

• Ensuring products or services meet global standards

• Navigating trade agreements and tariffs

• Managing risks associated with regulatory differences

Resin binding capacity refers to the maximum amount of resin that can be bound to a substrate.

• Resin binding capacity is influenced by factors such as surface area, porosity, and chemical composition of the substrate.

• It is important to optimize resin binding capacity to ensure efficient production processes.

• Examples of substrates with high resin binding capacity include activated carbon and ion exchange resins.

Good manufacturing practices are guidelines and procedures that ensure products are consistently produced and controlled according to quality standards.

• Ensure cleanliness and hygiene in the production area

• Follow standard operating procedures for all processes

• Regularly calibrate and maintain equipment

• Train staff on proper manufacturing practices

• Document all production activities for traceability

The plant was running smoothly and efficiently.

• The plant was operating at full capacity

• All equipment was functioning properly

• Regular maintenance and checks were being conducted

• Water quality was meeting all standards and regulations

WFI generation system stands for Water for Injection generation system, which is used in pharmaceutical industry to produce highly purified water for use in drug manufacturing.

• WFI generation systems use various methods such as distillation, reverse osmosis, and ultrafiltration to remove impurities from water.

• The purified water produced by WFI generation systems must meet strict quality standards set by regulatory agencies like the FDA.

• WFI generation systems are essential in p...read more

RO plant stands for Reverse Osmosis plant, a water treatment system that removes impurities from water by using a partially permeable membrane.

• RO plant uses reverse osmosis process to remove contaminants from water

• It works by applying pressure to the water, forcing it through a semi-permeable membrane

• The membrane allows only water molecules to pass through, leaving behind impurities

• RO plants are commonly used for desalination, wastewater treatment, and producing drinking wate...read more

Column packing is a process of filling a chromatography column with a stationary phase.

• Choose an appropriate stationary phase based on the separation requirements.

• Prepare the column by cleaning and conditioning it.

• Pack the column by carefully adding the stationary phase.

• Ensure proper packing density and uniformity.

• Test the packed column for efficiency and performance.

Column chromatography is a technique used to separate and purify components of a mixture based on their different affinities for a stationary phase and a mobile phase.

• Column chromatography involves passing a mixture through a column packed with a stationary phase, such as silica gel or alumina.

• The components of the mixture interact differently with the stationary phase, leading to their separation.

• The mobile phase, typically a solvent, carries the components through the colum...read more