What is hplc and polar and nonpolar

Question

Accepted Answer

HPLC is a technique used in analytical chemistry to separate, identify, and quantify components in a mixture. Polar and nonpolar refer to the polarity of molecules.

HPLC stands for High-Performance Liquid Chromatography.
It is a widely used technique in pharmaceutical, chemical, and food industries.
HPLC separates components in a mixture based on their interactions with a stationary phase and a mobile phase.
The stationary phase is a solid or liquid material packed into a column, while the mobile phase is a liquid solvent.
Polar molecules have an uneven distribution of charge and interact with polar solvents, while nonpolar molecules have an even distribution of charge and interact with nonpolar solvents.
Polar solvents include water, methanol, and ethanol, while nonpolar solvents include hexane, toluene, and chloroform.
The polarity of a molecule affects its retention time in HPLC.
By adjusting the composition of the mobile phase, different components can be separated and analyzed.
HPLC is used for drug analysis, environmental monitoring, quality control, and research purposes.

Accepted Answer

High performance liquid chromatography  polar- silicagel nonpolar-c8 are c18

What is hplc and polar and nonpolar

Popular interview questions of QC Officer

Top HR questions asked in Gland Pharma QC Officer