What is the difference between high pressure gradiant and low pressure gradiant in hplc

Question

Accepted Answer

High pressure gradient and low pressure gradient in HPLC differ in terms of the pressure applied to the mobile phase.

High pressure gradient involves applying a higher pressure to the mobile phase, resulting in faster elution and shorter run times.
Low pressure gradient involves applying a lower pressure to the mobile phase, resulting in slower elution and longer run times.
High pressure gradient is often used for rapid separations, while low pressure gradient is used for more complex separations.
The choice between high and low pressure gradient depends on the specific requirements of the HPLC analysis.

Accepted Answer

Hight presure gradiant is mobile phase is first pumping and next mixing but low pressure gradiant first mixing and after pumping

Accepted Answer

Hight presure gradiant is mobile phase is first pumping and next mixing but low pressure gradiant first mixing and after pumping

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