What is the difference between Gc and hplc

Question

Accepted Answer

GC and HPLC are both analytical techniques used in chemistry, but they differ in their principles and applications.

GC stands for Gas Chromatography, while HPLC stands for High-Performance Liquid Chromatography.
GC separates and analyzes volatile compounds based on their vaporization and partitioning between a stationary phase and a mobile gas phase.
HPLC separates and analyzes non-volatile compounds based on their partitioning between a stationary phase and a mobile liquid phase.
GC is commonly used for analyzing volatile organic compounds, such as hydrocarbons, in environmental, forensic, and pharmaceutical applications.
HPLC is often used for analyzing non-volatile organic compounds, such as drugs, pesticides, and biomolecules, in various industries including pharmaceutical, food, and environmental.
GC typically requires a gaseous mobile phase, such as helium or nitrogen, while HPLC uses a liquid mobile phase, such as water or organic solvents.
GC generally provides faster analysis times and higher separation efficiencies compared to HPLC.
Both techniques utilize detectors to measure the analyte concentration, such as flame ionization detector (FID) for GC and UV-Vis detector for HPLC.

Accepted Answer

HPLC uses a solvent for the mobile phase wherethe polarity, solubility and complexity of the compounds in the determine which solvent should be used.On the other hand, GC uses an unreactive or inert gas,also known as carrier gas

Accepted Answer

Gc is the gas chromatography this is the depends upon partition coofficient and separation of components into individual components with out dicompositikn without fail. And hplc is reversevphase depends upon partition and normal phase depends absorption the separation of individual compounds.

Accepted Answer

Gc is the gas chromatography this is the depends upon partition coofficient and separation of components into individual components with out dicompositikn without fail. And hplc is reversevphase depends upon partition and normal phase depends absorption the separation of individual compounds.

What is the difference between Gc and hplc

Top Biophore India Pharmaceuticals Senior Executive interview questions & answers

Popular interview questions of Senior Executive

Top HR questions asked in Biophore India Pharmaceuticals Senior Executive