Working principle of Spectrophotometer

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Spectrophotometer measures the amount of light absorbed by a sample at different wavelengths.

Spectrophotometer uses a light source to shine light on a sample and measures the amount of light absorbed by the sample at different wavelengths.
The amount of light absorbed is proportional to the concentration of the sample.
Spectrophotometer can be used to measure the concentration of a wide range of substances, including DNA, proteins, and chemicals.
UV-Vis spectrophotometer is commonly used in research and clinical laboratories.
Spectrophotometer can also be used to determine the purity of a sample by measuring the ratio of absorbance at different wavelengths.

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