Molecular Technician Interview Questions and Answers

PCR is a technique used to amplify DNA sequences. It involves three main steps: denaturation, annealing, and extension.

• Denaturation: The DNA sample is heated to separate the double-stranded DNA into single strands.

• Annealing: Primers bind to the complementary sequences on the DNA strands.

• Extension: DNA polymerase synthesizes new DNA strands using the primers as a starting point.

• Repeat: The process is repeated multiple times to amplify the DNA exponentially.