Calibration parameters of HPLC include wavelength, flow rate, injection volume, column temperature, and mobile phase composition.

• Wavelength: Determines the detection sensitivity and selectivity of the...read more

There are a total 9 parameters for HPLC calibration, these are as follows;

1. Flow Rate Accuracy

2. Injector Precision

3. Injector Linearity

4. Wavelength accuracy of the detector

5. Detector Linearity

6. Gradient Composition Accuracy

7. Carry Over

8. Temperature Accuracy

9. Drift and Noise