Powell Laboratories Accounts Officer Interview Questions and Answers

Analytical method validation is the process of demonstrating that an analytical procedure is suitable for its intended purpose.

• Validation ensures accuracy, precision, specificity, and linearity of an analytical method.

• Parameters validated include accuracy, precision, specificity, detection limit, quantitation limit, linearity, range, and robustness.

• Validation is typically done through experiments, comparison with a ref...read more

Analytical method development involves creating and optimizing methods to accurately analyze samples.

• Identifying the purpose of the analysis

• Selecting appropriate analytical techniques

• Optimizing parameters for accurate results

• Validating the method for reliability

• Documenting the method for reproducibility

QMS stands for Quality Management System, a set of policies, processes, and procedures required for planning and execution in the core business area of an organization.

• QMS ensures that products and services meet customer requirements and quality standards.

• It involves quality planning, quality control, quality assurance, and quality improvement.

• QMS helps in achieving consistency and improvement in operations.

• ISO 9001 is...read more

A pH meter is a scientific instrument used to measure the acidity or alkalinity of a substance. Calibration ensures accurate readings.

• pH meter measures the concentration of hydrogen ions in a solution

• Calibration involves adjusting the pH meter to known standard solutions

• Regular calibration ensures accurate readings over time

HPLC stands for High Performance Liquid Chromatography, which consists of a pump, injector, column, detector, and integrator.

• HPLC involves a pump to push the liquid solvent through the system.

• It includes an injector to introduce the sample into the mobile phase.

• The column separates the components of the sample based on their interactions with the stationary phase.

• A detector measures the amount of analyte in the eluent.

• ...read more

Buffers commonly used in HPLC include phosphate buffers, acetate buffers, and citrate buffers.

• Phosphate buffers are commonly used in HPLC for separating acidic compounds.

• Acetate buffers are used for separating basic compounds.

• Citrate buffers are used for separating metal ions.

• Other common buffers used in HPLC include ammonium acetate and ammonium formate.

HPLC calibration involves adjusting parameters to ensure accurate and reliable results.

• Prepare standard solutions of known concentrations

• Run the standard solutions through the HPLC system

• Compare the results to expected values and adjust parameters as needed

• Repeat the process until accurate and consistent results are obtained