Concept College Of Science And Technology Chakdara Interview Questions and Answers

Common acids and bases in labs include hydrochloric acid, sodium hydroxide, sulfuric acid, acetic acid, and ammonia.

• Hydrochloric Acid (HCl): A strong acid used for pH adjustment and cleaning.

• Sodium Hydroxide (NaOH): A strong base used in titrations and as a cleaning agent.

• Sulfuric Acid (H2SO4): A strong acid used in battery production and as a dehydrating agent.

• Acetic Acid (CH3COOH): A weak acid used in buffer solutions and food preservation.

• Ammonia (NH3): A weak base used in...read more

Blood collection vials are specialized containers used to collect, store, and transport blood samples for laboratory testing.

• Used for various blood tests, including CBC, blood chemistry, and serology.

• Different colors indicate specific additives, e.g., red for serum, blue for coagulation tests.

• Vacutainer system allows for easy blood collection with minimal contamination.

• Some vials are designed for specific tests, like EDTA vials for hematology.

GMP area classification categorizes environments based on cleanliness and contamination control in pharmaceutical manufacturing.

• Classified into different grades (e.g., A, B, C, D) based on the level of cleanliness required.

• Grade A areas are sterile, used for aseptic processing (e.g., filling vials).

• Grade B areas support Grade A, with controlled environments for critical operations.

• Grade C and D areas have less stringent requirements, used for less critical processes.

• Classific...read more

ELISA is a laboratory technique used to detect and quantify proteins, antibodies, and hormones in a sample.

• Types of ELISA include: Direct ELISA, Indirect ELISA, Sandwich ELISA, and Competitive ELISA.

• Direct ELISA: Detects antigens directly using enzyme-linked antibodies.

• Indirect ELISA: Involves a two-step process to detect antibodies against an antigen.

• Sandwich ELISA: Uses two antibodies to capture and detect the target antigen.

• Competitive ELISA: Measures the concentration of ...read more

Buffers in ELISA maintain pH and ionic strength for optimal antigen-antibody interactions.

• Coating buffer: Typically a carbonate-bicarbonate buffer (pH 9.6) for antigen attachment.

• Blocking buffer: Commonly BSA (Bovine Serum Albumin) or non-fat dry milk in PBS to prevent non-specific binding.

• Washing buffer: PBS or TBS with Tween-20 to remove unbound components while maintaining stability.

• Dilution buffer: Often PBS or specific assay buffers for diluting samples and reagents.