Types of Chromatographic techniques and their principle.

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Accepted Answer

Chromatographic techniques separate mixtures into individual components based on their physical and chemical properties.

Gas chromatography separates volatile compounds based on their boiling points and affinity for the stationary phase.
Liquid chromatography separates compounds based on their polarity and affinity for the stationary phase.
Ion exchange chromatography separates charged molecules based on their charge and affinity for the stationary phase.
Size exclusion chromatography separates molecules based on their size and shape.
Affinity chromatography separates molecules based on their specific binding interactions with the stationary phase.
Paper chromatography separates compounds based on their solubility and affinity for the paper.
Thin layer chromatography separates compounds based on their polarity and affinity for the stationary phase.
High performance liquid chromatography (HPLC) is a type of liquid chromatography that uses high pressure to increase separation efficiency.
Gas chromatography-mass spectrometry (GC-MS) combines gas chromatography with mass spectrometry to identify individual components in a mixture.
Liquid chromatography-mass spectrometry (LC-MS) combines liquid chromatography with mass spectrometry to identify individual components in a mixture.

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