Gram staining,cell disruption process? What are the factors which shows fermentation complete?

Question

Accepted Answer

Gram staining is a technique used to differentiate bacteria based on their cell wall composition. Cell disruption is a process to break open cells to release their contents.

Gram staining involves staining bacterial cells with crystal violet dye, followed by iodine treatment and decolorization with alcohol or acetone.
Gram-positive bacteria retain the crystal violet stain and appear purple, while Gram-negative bacteria lose the stain and appear pink or red after counterstaining with safranin.
Cell disruption methods include mechanical disruption (e.g., sonication, bead beating), enzymatic digestion, and chemical disruption (e.g., detergents, organic solvents).
Factors indicating fermentation completion include the absence of glucose, production of acid or gas, and changes in pH or color of the medium.
For example, in the fermentation of glucose by yeast, the absence of glucose and the presence of alcohol and carbon dioxide indicate fermentation completion.

Gram staining,cell disruption process? What are the factors which shows fermentation complete?

Top Panacea Biotec Scientific Officer interview questions & answers

Popular interview questions of Scientific Officer

Top HR questions asked in Panacea Biotec Scientific Officer