Panacea Biotec Scientific Officer Interview Questions and Answers

Gram staining is a technique used to differentiate bacteria based on their cell wall composition. Cell disruption is a process to break open cells to release their contents.

• Gram staining involves staining bacterial cells with crystal violet dye, followed by iodine treatment and decolorization with alcohol or acetone.

• Gram-positive bacteria retain the crystal violet stain and appear purple, while Gram-negative bacteria lose the stain and appear pink or red after counterstaining...read more

Chromatographic techniques separate mixtures into individual components based on their physical and chemical properties.

• Gas chromatography separates volatile compounds based on their boiling points and affinity for the stationary phase.

• Liquid chromatography separates compounds based on their polarity and affinity for the stationary phase.

• Ion exchange chromatography separates charged molecules based on their charge and affinity for the stationary phase.

• Size exclusion chromatog...read more

Laminar Air Flow and Biosafety Cabinet are both used to maintain sterile environments, but have different purposes.

• Laminar Air Flow is used to maintain a sterile environment for non-hazardous materials, such as in a laboratory or clean room.

• Biosafety Cabinet is used to maintain a sterile environment for hazardous materials, such as in a medical or research setting.

• Laminar Air Flow blows filtered air in a single direction, while Biosafety Cabinet has HEPA filters and exhaust s...read more

Area classification refers to the process of categorizing areas based on their potential for the presence of hazardous substances or environments.

• Area classification is important for ensuring safety in industries such as oil and gas, chemical manufacturing, and mining.

• It involves assessing the likelihood and extent of the presence of flammable gases, vapors, or combustible dusts.

• Classification is typically based on factors such as the type and quantity of hazardous substances...read more

Types of media used in laboratory include agar, broth, and selective media.

• Agar is a solid medium used for culturing microorganisms.

• Broth is a liquid medium used for growing bacteria and other microorganisms.

• Selective media contains specific nutrients or chemicals to promote the growth of certain types of microorganisms.

• Other types of media include enriched media, differential media, and transport media.

Thesis or dissertation procedure in masters

• A thesis or dissertation is a research project that students undertake as part of their master's degree program

• The procedure involves selecting a topic, conducting research, analyzing data, and presenting findings

• The thesis or dissertation is typically supervised by a faculty member and must meet certain formatting and citation requirements

• The final product is a written document that demonstrates the student's mastery of the subject ...read more

A controlled area is a restricted area where access is limited and certain safety measures are enforced.

• Access to a controlled area is restricted to authorized personnel only

• Safety measures such as protective clothing, equipment, and procedures are enforced

• Examples include nuclear power plants, chemical manufacturing facilities, and research laboratories

• Controlled areas are established to protect people, property, and the environment from potential hazards

IPQA stands for In-Process Quality Assurance. LAF stands for Laminar Air Flow.

• IPQA is a quality control process that ensures that products are manufactured according to the required standards.

• It involves monitoring and verifying the manufacturing process at various stages to ensure that the product meets the required specifications.

• LAF is a type of air flow system used in clean rooms and laboratories to maintain a sterile environment.

• It works by filtering the air and directin...read more