What are the differences between Real-Time PCR and conventional PCR?

Question

Accepted Answer

Real-Time PCR allows for monitoring the amplification of DNA in real-time, while conventional PCR requires post-amplification analysis.

Real-Time PCR provides quantitative data in real-time, while conventional PCR only provides qualitative data after the reaction is complete.
Real-Time PCR uses fluorescent dyes or probes to monitor amplification as it occurs, while conventional PCR relies on gel electrophoresis to visualize the final products.
Real-Time PCR is more sensitive and specific than conventional PCR, making it ideal for applications such as gene expression analysis and pathogen detection.
Examples of Real-Time PCR machines include the Applied Biosystems QuantStudio and the Bio-Rad CFX systems.
Conventional PCR is more cost-effective and easier to set up compared to Real-Time PCR, but lacks the ability to provide real-time data.

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