Entrans Technologies Interview Questions and Answers

Nucleic acids are biomolecules essential for storing and transmitting genetic information in cells.

• Nucleic acids are made up of nucleotides, which consist of a sugar, a phosphate group, and a nitrogenous base.

• There are two types of nucleic acids: DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).

• DNA carries genetic information and is responsible for inheritance, while RNA plays a role in protein synthesis.

• Nucleic acids are crucial for the replication and expression of ge...read more

PCR is a technique used to amplify a specific DNA sequence, producing millions of copies for analysis.

• PCR involves cycles of heating and cooling to denature, anneal, and extend DNA strands.

• Types of PCR include conventional PCR, quantitative PCR (qPCR), reverse transcription PCR (RT-PCR), and digital PCR.

• Conventional PCR is used for DNA amplification, qPCR for quantifying DNA, RT-PCR for RNA amplification, and digital PCR for absolute quantification.

Nucleic acids are macromolecules made up of nucleotides, while nucleosides are smaller molecules composed of a nitrogenous base and a sugar.

• Nucleic acids are polymers made up of nucleotides, which consist of a nitrogenous base, a sugar, and a phosphate group.

• Examples of nucleic acids include DNA and RNA.

• Nucleosides are smaller molecules composed of a nitrogenous base and a sugar, but they lack the phosphate group found in nucleotides.

• Examples of nucleosides include adenosine ...read more

Real-Time PCR allows for monitoring the amplification of DNA in real-time, while conventional PCR requires post-amplification analysis.

• Real-Time PCR provides quantitative data in real-time, while conventional PCR only provides qualitative data after the reaction is complete.

• Real-Time PCR uses fluorescent dyes or probes to monitor amplification as it occurs, while conventional PCR relies on gel electrophoresis to visualize the final products.

• Real-Time PCR is more sensitive and...read more

DNA extraction and isolation is the process of obtaining DNA from cells for further analysis.

• Cells are lysed to release DNA

• Proteins and other contaminants are removed through precipitation or column purification

• DNA is then precipitated and washed to remove impurities

• Finally, DNA is resuspended in a buffer for storage or analysis

Purines and pyrimidines are types of nitrogenous bases found in DNA and RNA molecules.

• Purines are double-ring structures (adenine, guanine)

• Pyrimidines are single-ring structures (cytosine, thymine, uracil)

• They are essential components of nucleic acids and play a crucial role in genetic information transfer