Why giving column condition during Hplc analysis?

Question

Accepted Answer

Column conditions are set to optimize separation and detection of analytes in HPLC analysis.

Column conditions include mobile phase composition, flow rate, column temperature, and column type.
Optimizing column conditions can improve resolution, sensitivity, and selectivity of the analysis.
Column conditions may vary depending on the analytes being analyzed and the purpose of the analysis.
Examples of column conditions include using a C18 column with a gradient of acetonitrile and water as the mobile phase.
Column conditions can also affect the lifespan of the column and the reproducibility of the analysis.

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