The stationary phase used in HPLC can be either polar or non-polar.

• Polar stationary phases are used for separating polar compounds, while non-polar stationary phases are used for separating non-polar ...read more

There are two types of chromatography Normal phase chromatography and Reverse phase chromatography.

According to chromatography stationary phases may differ.

For Normal Phase: During normal-phase chromatography, the mobile phase is 100% organic. Only trace amounts of water and polar packing particles exist in the mobile phase. Polar analytes bind strongly to the polar stationary phase and may be rendered non-elutable.

The stationary phase is more polar than the mobile phase is called a normal phase.

Stationary phase: The term reversed-phase describes this chromatography mode, which is the opposite of the normal phase, where a polar mobile phase is used with a non-polar [hydrophobic] stationary phase.

The mobile phase is more polar than the stationary phase is called a Reverse Phase.

The typical stationary phase for HPLC can be silica-based or polymer-based

The stationary phase used in HPLC is silica-based or polymer-based