Legency Remedies (Pvt.)
OYO Life Interview Questions and Answers
Q1. Different between Procaryotic and eucaryotic cell?
Procaryotic cells lack a nucleus and membrane-bound organelles, while eucaryotic cells have both.
Procaryotic cells are smaller and simpler in structure than eucaryotic cells.
Procaryotic cells have a single circular chromosome, while eucaryotic cells have multiple linear chromosomes.
Procaryotic cells have cell walls made of peptidoglycan, while eucaryotic cells have cell walls made of cellulose or chitin.
Examples of procaryotic cells include bacteria and archaea, while example...read more
Q2. Full form of HEPA filter and it's pore size?
HEPA stands for High Efficiency Particulate Air. The pore size of a HEPA filter is typically 0.3 micrometers.
HEPA stands for High Efficiency Particulate Air.
HEPA filters are used to remove particles and contaminants from the air.
The pore size of a HEPA filter is typically 0.3 micrometers.
HEPA filters are commonly used in cleanrooms, hospitals, and HVAC systems.
Q3. Why we are using only 70% IPA?
70% IPA is used as it is more effective in killing microorganisms than higher concentrations.
70% IPA has a higher water content which allows it to penetrate the cell wall of microorganisms more effectively
Higher concentrations of IPA can cause coagulation of proteins on the surface of microorganisms, making them more resistant to disinfection
70% IPA is less volatile than higher concentrations, allowing for longer contact time with the surface being disinfected
Q4. Process of Gram staining step by step?
Gram staining is a technique used to differentiate bacteria into two groups: Gram-positive and Gram-negative.
Prepare a heat-fixed bacterial smear on a glass slide
Cover the smear with crystal violet stain for 1 minute
Rinse the slide with water to remove excess stain
Apply iodine solution to the slide for 1 minute
Rinse the slide with water again
Decolorize the slide with alcohol or acetone for a few seconds
Rinse the slide with water once more
Counterstain the slide with safranin f...read more
Q5. Principle of Autoclave and it's stages?
Autoclave uses steam under pressure to sterilize equipment and materials.
Autoclave operates at high temperature and pressure to kill microorganisms
Three stages of autoclaving: heating, sterilization, and cooling
Heating stage: steam is introduced into the chamber to raise the temperature and pressure
Sterilization stage: temperature and pressure are maintained for a set time to kill microorganisms
Cooling stage: pressure is released and the chamber is cooled before opening
Autocl...read more
Q6. Full form of ACE-2 receptor and their formation?
ACE-2 receptor stands for Angiotensin-Converting Enzyme 2 receptor. It is a protein that is found on the surface of many cell types.
ACE-2 receptor is formed by the ACE2 gene.
It is primarily found in the lungs, heart, kidneys, and intestines.
ACE-2 receptor plays a crucial role in regulating blood pressure and cardiovascular function.
It is also the receptor that allows the SARS-CoV-2 virus to enter human cells and cause COVID-19.
ACE-2 receptor is a potential target for developi...read more
Q7. Constituents of SCDM and FTGM media?
SCDM and FTGM are culture media used for microbial growth and identification.
SCDM stands for Sabouraud Dextrose Chloramphenicol Medium and is used for fungal isolation and identification.
FTGM stands for Fluid Thioglycollate Medium and is used for anaerobic bacterial growth.
SCDM contains dextrose, peptone, chloramphenicol, and agar.
FTGM contains thioglycollate, dextrose, cysteine, and agar.
Both media are commonly used in microbiology laboratories for microbial culture and iden...read more
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