What is HPLC/principle/instrumentation/normal and reverse phase/detectors

Question

Accepted Answer

HPLC is a technique used to separate, identify, and quantify components in a mixture based on their interaction with a stationary phase and mobile phase.

HPLC stands for High-Performance Liquid Chromatography.
The principle of HPLC involves the separation of components in a mixture based on their affinity for the stationary phase and mobile phase.
The instrumentation of HPLC includes a pump to deliver the mobile phase, a sample injector, a column containing the stationary phase, and a detector to measure the separated components.
Normal phase HPLC uses a polar stationary phase and a non-polar mobile phase, while reverse phase HPLC uses a non-polar stationary phase and a polar mobile phase.
Detectors used in HPLC include UV-Vis detectors, fluorescence detectors, and mass spectrometers.

Accepted Answer

High performance liquid chromatography

Basic principles separation

Normal phase non polar stationary phase and reverse phase polar mobile phase

And non polar stationary phase

Detector photo diode array used

Accepted Answer

High performance liquid chromatograph

What is HPLC/principle/instrumentation/normal and reverse phase/detectors

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